文献类型： 外文期刊

作者： Hu, X. F. ¹ ; Guo, Y. M. ¹ ; Huang, B. Y. ¹ ; Bun, S. ¹ ; Zhang, L. B. ¹ ; Li, J. H. ¹ ; Liu, D. ¹ ; Long, F. Y. ¹ ; Yang, X ¹ ;

作者机构： 1.China Agr Univ, State Key Lab Anim Nutr, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

2.Henan Acad Agr Sci, Henan Key Lab

关键词： DNA;RNA;saline: 31661-12-8;total protein;glucagon-like peptide 2: GLP-2;89750-15-2;mRNA;corticosterone: CORT;50-22-6;vitamin D-dependent calcium binding protein;peptide transporter 1;liver fatty acid-binding protein: L-FABP;nutrie;nt transporter: expression;sodium and glucose co-transporter 1: SGLT-1;growth performance;small intestinal morphology;feed intake ratio;relative small intestinal weight

期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）

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收录情况： SCI

摘要： An experiment was conducted to determine the effect of injecting glucagon-like peptide 2 (GLP-2) on the small intestinal weight, morphology, and nutrient transporter expression in pharmacologically stressed broiler chickens. A total of 144 seven-day-old birds were fed either a basal diet (CTRL) or a basal diet plus 30 mg of corticosterone (CORT)/kg of diet for a total of 14 d. Half of the birds from each group were injected daily with GLP-2 (6.7 nmol/kg of BW) or saline for 14 d. The average final BW, ADG, ADFI, and the ratio of feed intake to weight gain (F: G) was recorded over 21 d for the 4 groups of 36 birds, namely CTRL + saline, CTRL + GLP-2, CORT + saline, and CORT + GLP-2. In addition, the absolute and relative small intestinal weight, villus height (VH), and crypt depth (CD) of the duodenum and jejunum, as well as the abundance of sodium and glucose co-transporter 1 (SGLT-1), vitamin D-dependent calcium-binding protein-28,000 molecular weight (CaBP-D28k), and peptide transporter 1 (PepT-1) mRNA in the duodenum and of liver fatty acid-binding protein (L-FABP) mRNA in the jejunum. The total DNA, RNA, and protein content in small intestinal mucosa were also determined. The results showed that CORT administration significantly lowered average final BW, ADG, ADFI, absolute small intestinal weight, VH, and CD of duodenum and jejunum (P < 0.05) while increasing the relative small intestinal weight, F: G, relative abundance of SGLT-1, CaBP-D28k, PepT-1, and L-FABP mRNA (P < 0.05). Glucagon-like peptide 2 injection increased the average final BW, ADG, VH, and CD in duodenum and jejunum and relative abundance of SGLT-1, CaBP-28k, PepT1, and PepT1 mRNA of broiler chickens, respectively (P < 0.05), and decreased F: G (P < 0.05). In chickens fed basal diet plus CORT, injecting GLP-2 decreased F: G (P < 0.05); increased VH and CD of duodenum and CD of jejunum; and increased relative abundance of SGLT1, CaBP-D28k, PepT-1, and L-FABP mRNA, RNA, and total protein content in small intestine compared with the injection of saline (P < 0.05). In birds fed the basal diet, GLP-2 injection decreased F: G (P < 0.05) and increased final BW, ADG, small bowel weight, CD of jejunum, and relative abundance of CaBP-D28k and PepT-1 mRNA compared with injecting saline (P < 0.05). In conclusion, GLP-2 injection reversed the negative effect of stress on the weight and morphology and the absorptive function of small bowel of broiler chickens. Glucagon-like peptide 2 injection also had a positive effect on the growth performance of healthy broiler chickens.