Novel antioxidant peptides from bovine blood: Purification, identification and mechanism of action
文献类型： 外文期刊
作者： Wang, Lin 1 ; Li, Ziyu 1 ; Fan, Xia 1 ; Zhang, Ting 2 ; Wang, Hui 3 ; Ye, Keping 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, State Key Lab Meat Qual Control & Cultured Meat De, Nanjing 210095, Peoples R China
2.Xinjiang Acad Agr Sci, State Inst Agr Prod Storage & Proc, Xinjiang 830091, Peoples R China
3.State Xinjiang Hualing Agr & Anim Husb Sci & Techn, Xinjiang 830063, Peoples R China
关键词： Bovine blood; Antioxidant peptide; Separation and purification; Identification; Keap1-Nrf2 signaling pathway
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）
ISSN： 0023-6438
年卷期： 2024 年 205 卷
页码：
收录情况： SCI
摘要： The purpose of this study was to isolate, purify, identify, and characterize novel antioxidant peptides derived from bovine blood hydrolysates (BBH). Results showed that, BBH, obtained by two-step flavourzyme-alcalase hydrolysis, was used to obtain five fractions by ultrafiltration and gel filtration chromatography, where the Fraction B had the strongest antioxidant capacity. Five novel antioxidant peptides (GFAGDDAPRA, SGPGIHVR, APSADAPM, TQRFFESF and HVDPENFKLL) were identified utilizing RP-HPLC and LC-MS/MS, and exhibited excellent free radical scavenging ability. GFAGDDAPRA and TQRFFESF possessed the highest ABTS radical scavenging rate (95.70% and 93.20%, respectively), and the DPPH radical scavenging rate of APSADAPM was the highest (50.28%). Moreover, these five peptides could be docked with Recombinant Kelch Like ECH Associated Protein 1 (Keap1) with the binding energies less than -5 kcal/mol, which indicated that they may be able to promote the body's antioxidant capacity by activating the Keap1-Nrf2 signaling pathway of cell. Meaningfully, this work shed new light on the processing and utilization of bovine blood, as well as offered a valuable foundation for the study and development of food-borne antioxidant peptides.
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