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The E3 ubiquitin ligase CSIT1 regulates critical sterility-inducing temperature by ribosome- associated quality control to safeguard two-line hybrid breeding in rice

文献类型： 外文期刊

作者： Peng, Guoqing 1 ; Liu, Minglong 1 ; Zhu, Liya 1 ; Luo, Wenlong 3 ; Wang, Qinghua 1 ; Wang, Mumei 1 ; Chen, Huiqiong 1 ; Luo, Ziliang 4 ; Xiao, Yueping 1 ; Zhang, Yongjie 1 ; Hong, Haona 1 ; Liu, Zhenlan 1 ; Zhou, Lingyan 2 ; Guo, Guoqiang 5 ; Wang, Yingxiang 1 ; Zhuang, Chuxiong 1 ; Zhou, Hai 1 ;

作者机构： 1.South China Agr Univ, Coll Life Sci, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangdong Lab Lingnan Modern Agr,Key Lab Enhancing, Guangzhou 510642, Peoples R China

2.Zhongkai Univ Agr & Engn, Coll Agr & Biol, Guangzhou 510225, Peoples R China

3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Peoples R China

4.Univ Florida, Agron Dept, Gainesville, FL 32610 USA

5.Hengyang Acad Agr Sci, Hengyang 421101, Peoples R China

关键词： hybrid breeding; thermo-sensitive genic male sterility; critical sterility-inducing temperature; ribo-some-associated quality control; programmed cell death

期刊名称：MOLECULAR PLANT （ 影响因子：27.5； 五年影响因子：22.6 ）

ISSN： 1674-2052

年卷期： 2023 年 16 卷 10 期

页码：

收录情况： SCI

摘要： Two-line hybrid breeding can fully utilize heterosis in crops. In thermo-sensitive genic male sterile (TGMS) lines, low critical sterility-inducing temperature (CSIT) is vital to safeguard the production of two-line hybrid seeds in rice (Oryza sativa), but the molecular mechanism determining CSIT is unclear. Here, we report the cloning of CSIT1, which encodes an E3 ubiquitin ligase, and show that CSIT1 modulates the CSIT of thermosensitive genic male sterility 5 (tms5)-based TGMS lines through ribosome-associated quality control (RQC). Biochemical assays demonstrated that CSIT1 binds to the 80S ribosomes and ubiquitinates abnormal nascent polypeptides for degradation in the RQC process. Loss of CSIT1 function inhibits the possible damage of tms5 to the ubiquitination system and protein translation, resulting in enhanced accumulation of anther-related proteins such as catalase to suppress abnormal accumulation of reactive oxygen species and premature programmed cell death in the tapetum, thereby leading to a much higher CSIT in the tms5-based TGMS lines. Taken together, our findings reveal a regulatory mechanism of CSIT, providing new insights into RQC and potential targets for future two-line hybrid breeding.

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