文献类型: 外文期刊
作者: Lu, Li 1 ; Liu, Jinxian 1 ; Zhang, Wenneng 1 ; Cheng, Xi 1 ; Zhang, Bo 1 ; Yang, Yiyang 4 ; Que, Youxiong 5 ; Li, Yuanhua 1 ; Li, Xinghui 6 ;
作者机构: 1.Wuyi Univ, Coll Tea & Food, Wuyishan 354300, Peoples R China
2.Tea Engn Res Ctr Fujian Higher Educ, Wuyishan 354300, Peoples R China
3.Wuyi Univ, Tea Sci Res Inst, Wuyishan 354300, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Leisure Agr, Nanjing 210014, Peoples R China
5.Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr & Rural Affairs, Fuzhou 350002, Peoples R China
6.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China
关键词: Wuyi black tea; processing; quality components; polyphenoloxidase; cellulase; gene expression
期刊名称:FOODS ( 影响因子:5.2; 五年影响因子:5.5 )
ISSN:
年卷期: 2024 年 13 卷 9 期
页码:
收录情况: SCI
摘要: As the most consumed tea in the world, all kinds of black tea are developed from Wuyi black tea. In this study, quality components, regulatory gene expression, and key enzyme activity during the processing were analyzed to illustrate the taste formation of WBT. Withering mainly affected the content of amino acids, while catechins and tea pigments were most influenced by rolling and the pre-metaphase of fermentation. Notably, regulatory gene expression was significantly down-regulated after withering except for polyphenoloxidase1, polyphenoloxidase2, leucoanthocyanidin dioxygenase, chalcone isomerase, and flavonoid 3 ', 5 '-hydroxylase. Co-expression of flavonoid pathway genes confirmed similar expression patterns of these genes in the same metabolic pathway. Interestingly, rolling and fermentation anaphase had a great effect on polyphenol oxidase, and fermentation pre-metaphase had the greatest effect on cellulase. Since gene regulation mainly occurs before picking, the influence of chemical reaction was greater during processing. It was speculated that polyphenol oxidase and cellulase, which promoted the transformation of quality components, were the key factors in the quality formation of WBT. The above results provide theoretical basis for the processing of WBT and the reference for producing high-quality black tea.
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