Development of a Rapid Sporulation Method of Fusarium graminearum Using Liquid Cultivation
文献类型： 外文期刊
作者： Zhang, Xue 1 ; Li, Chunjie 2 ; Xue, Baiyan 1 ; Ji, Pingsheng 3 ; Li, Yonggang 1 ; Sun, Lei 4 ; Wang, Shuang 4 ;
作者机构： 1.Northeast Agr Univ, Agr Coll, Harbin 150030, Heilongjiang, Peoples R China
2.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin 150081, Heilongjiang, Peoples R China
3.Univ Georgia, Dept Plant Pathol, Tifton, GA 31794 USA
4.Heilongjiang Acad Agr Sci, Inst Soil Fertilizer & Environm Resources, Harbin 150086, Heilongjiang, Peoples R China
关键词： conidia; culture conditions; Fusarium graminearum; liquid cultivation
期刊名称：PLANT DISEASE （ 影响因子：4.614； 五年影响因子：5.33 ）
ISSN： 0191-2917
年卷期： 2022 年 106 卷 1 期
页码：
收录情况： SCI
摘要： Fusarium graminearum is an important fungus causing a variety of maize diseases, including stalk rot, ear rot, and sheath rot. However, conidia of F. graminearum are not easily obtained under normal culture conditions, which seriously affects the identification and pathogenicity assessment of the isolates and screening of resistance sources. This study was undertaken to develop and utilize a rapid sporulation technique of F. graminearum using liquid cultivation, which could meet the needs of various tests. The results show that the optimum conditions for sporulation of F. graminearum were as follows: culture medium, 0.154 mol/liter of saline; temperature, 28 to 30 degrees C; incubation time, 96 h; initial pH, 9 to 10; illumination, continuous ultraviolet light; and shaking speed, 150 rpm. Using this culture method, conidial concentration of tested F. graminearum strains can reach >1.5 x 10(5) conidia/ml. Compared with the existing methods using mung bean and carboxylmethyl cellulose as matrix, saline is relatively inexpensive, and the culture process, relatively quick. Overall, this study provided a systematic, rapid, and simple method to obtain a large number of conidia of F. graminearum.
- 相关文献
作者其他论文 更多>>
-
Response of Crop Yield and Productivity Contribution Rate to Long-Term Different Fertilization in Northeast of China
作者：Ma, Xingzhu;Ma, Xingzhu;Hao, Xiaoyu;Zhao, Yue;Ji, Jinghong;Liu, Shuangquan;Zheng, Yu;Sun, Lei;Zhou, Baoku;Ma, Xingzhu;Hao, Xiaoyu;Zhao, Yue;Zheng, Yu;Ma, Xingzhu;Hao, Xiaoyu;Zhao, Yue;Ji, Jinghong;Liu, Shuangquan;Zheng, Yu;Peng, Xinhua
关键词：black soil; long-term fertilization; yield sustainability; rotatioin; contribution rate of productivity
-
Identification, Characterization, and Chemical Management of Fusarium asiaticum Causing Soybean Root Rot in Northeast China
作者：Liu, Jinxin;Cui, Wanqiu;Zhao, Qingyi;Li, Yonggang;Ren, Zhipeng;Li, Lin;Sun, Lei;Ding, Junjie
关键词：soybean; root rot;
Fusarium asiaticum ; host range; identification; fungicide efficacy -
Combined effects of cropping alfalfa (Medicago sativa L.) on the soil pore structure, microbial communities and organic carbon fractions in saline soils
作者：Zhu, Dan;Sun, Lei;Mao, Lina;Yan, Bohan;Li, Bin;Li, Xin;Li, Jingyang;Li, Bin;Li, Xin
关键词：Phytoremediation; Computed tomography scanning; Organic carbon fractions; Microorganisms; Co-occurrence network
-
Evaluation of Bacillus amyloliquefaciens isolate BX13 for the biocontrol of Fusarium incarnatum, a causal agent of fruit rot in postharvest muskmelon
作者：Xu, Chuzhen;Liu, Jinxin;Zhang, Xue;Li, Yonggang;Xu, Chuzhen;You, Haibo;Jia, Yunhe;Wang, Xiqing
关键词：biological control;
Bacillus amyloliquefaciens ;Fusarium incarnatum ; fruit rot; muskmelon; postharvest disease -
Evaluation of Bacillus amyloliquefaciens isolate BX13 for the biocontrol of Fusarium incarnatum, a causal agent of fruit rot in postharvest muskmelon
作者：Xu, Chuzhen;Liu, Jinxin;Zhang, Xue;Li, Yonggang;Xu, Chuzhen;Jia, Yunhe;You, Haibo;Wang, Xiqing
关键词：biological control; Bacillus amyloliquefaciens; Fusarium incarnatum; fruit rot; muskmelon; postharvest disease
-
Adaptability evaluation of four alfalfa (Medicago sativa L.) varieties in cold regions of northern China: Based on multidimensional indicators, metabolites, and soil microbial community analysis
作者：He, Ying;Zhao, Yuzi;Niu, Jiayi;Tian, Lijuan;Li, Yonggang;Li, Jiekai;Wang, Ziyu;Wu, Jianzhong;Han, Weibo
关键词：Medicago sativa; Cold adaptation; Multidimensional evaluation framework; Rhizosphere microbiome-metabolome crosstalk; Fuzzy membership analysis
-
Transcriptomic and Metabolomic Explanation of the Interaction between Soybean and Root Rot Caused by Fusarium tricinctum
作者：Zhang, Xue;Liu, Jinxin;Zhang, Ruisi;Li, Yonggang;Li, Jinrong;Wang, Jiajun;Li, Jiekai;Zhang, Dongmei
关键词：soybean root rot;
Fusarium tricinctum ; metabolome; chlorogenic acid; transcriptome
