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Development of a Rapid Sporulation Method of Fusarium graminearum Using Liquid Cultivation

文献类型： 外文期刊

作者： Zhang, Xue 1 ; Li, Chunjie 2 ; Xue, Baiyan 1 ; Ji, Pingsheng 3 ; Li, Yonggang 1 ; Sun, Lei 4 ; Wang, Shuang 4 ;

作者机构： 1.Northeast Agr Univ, Agr Coll, Harbin 150030, Heilongjiang, Peoples R China

2.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin 150081, Heilongjiang, Peoples R China

3.Univ Georgia, Dept Plant Pathol, Tifton, GA 31794 USA

4.Heilongjiang Acad Agr Sci, Inst Soil Fertilizer & Environm Resources, Harbin 150086, Heilongjiang, Peoples R China

关键词： conidia; culture conditions; Fusarium graminearum; liquid cultivation

期刊名称：PLANT DISEASE （ 影响因子：4.614； 五年影响因子：5.33 ）

ISSN： 0191-2917

年卷期： 2022 年 106 卷 1 期

页码：

收录情况： SCI

摘要： Fusarium graminearum is an important fungus causing a variety of maize diseases, including stalk rot, ear rot, and sheath rot. However, conidia of F. graminearum are not easily obtained under normal culture conditions, which seriously affects the identification and pathogenicity assessment of the isolates and screening of resistance sources. This study was undertaken to develop and utilize a rapid sporulation technique of F. graminearum using liquid cultivation, which could meet the needs of various tests. The results show that the optimum conditions for sporulation of F. graminearum were as follows: culture medium, 0.154 mol/liter of saline; temperature, 28 to 30 degrees C; incubation time, 96 h; initial pH, 9 to 10; illumination, continuous ultraviolet light; and shaking speed, 150 rpm. Using this culture method, conidial concentration of tested F. graminearum strains can reach >1.5 x 10(5) conidia/ml. Compared with the existing methods using mung bean and carboxylmethyl cellulose as matrix, saline is relatively inexpensive, and the culture process, relatively quick. Overall, this study provided a systematic, rapid, and simple method to obtain a large number of conidia of F. graminearum.

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