Induction of tetraploid via somatic embryos treated with colchicine in Hevea brasiliensis (Willd. ex A.Juss.) Mull. Arg
文献类型: 外文期刊
作者: Zhang, Yuanyuan 1 ; Wang, Xiangjun 1 ; Huang, Xiao 1 ; Li, Weiguo 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou 571101, Hainan, Peoples R China
2.State Ctr Rubber Breeding, Haikou 571101, Hainan, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Biol & Genet Resources Rubber Tree, Haikou 571101, Hainan, Peoples R China
关键词: Hevea brasiliensis; Tetraploid; Somatic embryogenesis; Phenotypic traits
期刊名称:PLANT CELL TISSUE AND ORGAN CULTURE ( 影响因子:2.726; 五年影响因子:2.859 )
ISSN: 0167-6857
年卷期: 2022 年 150 卷 2 期
页码:
收录情况: SCI
摘要: Polyploidy is a potential way to breed new varieties with high yield and stress resistance in plants. In this study, we provided an efficient method for induction of tetraploid of Hevea brasiliensis by immerse somatic embryos in the liquid plant induction medium containing colchicine. The optimum concentration of colchicine is 5 mg/ml, and the optimum immersion time is 24-48 h. The highest average induction rate of tetraploid is 27.12% in Reyan73397 with 5 mg/ml colchicine for 48 h, and 38.89% in Reyan87662 with 5 mg/ml colchicine for 24 h. Finally, 82 tetraploids of Reyan73397 and 14 tetraploids of Reyan87662 were obtained. Compared to diploids, tetraploids exhibited less and lighter leaf veins, daker and thicker leaves, larger stomatal size and smaller stomatal density. These tetraploids have the potential to be used as parents to produce new triploid germplasms in Hevea brasiliensis breeding programs.
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