Single-Cell Sequencing and Organoids: A Powerful Combination for Modelling Organ Development and Diseases
文献类型： 外文期刊
作者： Yin, Yuebang 1 ; Liu, Peng-Yu 2 ; Shi, Yinghua 1 ; Li, Ping 4 ;
作者机构： 1.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou, Peoples R China
2.Erasmus MC Univ Med Ctr, Dept Gastroenterol & Hepatol, Rotterdam, Netherlands
3.Southern Med Univ, Shenzhen Hosp, Clin Innovat & Res Ctr, Shenzhen Key Lab Viral Oncol, Shenzhen, Guangdong, Peoples R China
4.State Key Lab Livestock & Poultry Breeding, Guangzhou, Peoples R China
5.Minist Agr, Key Lab Anim Nutr & Feed Sci South China, Guangzhou, Peoples R China
6.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou, Peoples R China
关键词： Development; Disease; Organoids; Single-cell sequencing
期刊名称：REVIEWS OF PHYSIOLOGY, BIOCHEMISTRY AND PHARMACOLOGY （ 影响因子：5.545； 五年影响因子：5.645 ）
ISSN： 0303-4240
年卷期： 2021 年 179 卷
页码：
收录情况： SCI
摘要： The development and function of a particular organ and the pathogenesis of various diseases remain intimately linked to the features of each cell type in the organ. Conventional messenger RNA- or protein-based methodologies often fail to elucidate the contribution of rare cell types, including some subpopulations of stem cells, short-lived progenitors and circulating tumour cells, thus hampering their applications in studies regarding organ development and diseases. The scRNA-seq technique represents a new approach for determining gene expression variability at the single-cell level. Organoids are new preclinical models that recapitulate complete or partial features of their original organ and are thought to be superior to cell models in mimicking the sophisticated spatiotemporal processes of the development and regeneration and diseases. In this review, we highlight recent advances in the field of scRNA-seq, organoids and their current applications and summarize the advantages of using a combination of scRNA-seq and organoid technology to model diseases and organ development.
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