文献类型: 外文期刊
作者: Wang, Xuming 1 ; Yang, Yong 1 ; Yu, Chulang 1 ; Zhou, Jie 1 ; Cheng, Ye 1 ; Yan, Chengqi 1 ; Chen, Jianping 1 ;
作者机构: 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, MOA, Hangzhou 310021, Zhejiang, Peoples R China; Zhejiang Acad Agr Sci, Zhejiang Prov Key Lab Plant Virol, Hangzhou 310021, Zhejiang, Peoples R China
关键词: Artificial miRNA;Gene silencing;Oryza sativa;Vector construction
期刊名称:MOLECULAR BIOTECHNOLOGY ( 影响因子:2.695; 五年影响因子:2.303 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Artificial microRNA (amiRNA) has become a powerful tool for gene silencing in plants. A new method for easy and rapid construction of rice artificial miRNA vector is described. The procedure involved modification of the pCAMBIA1300-UR vector by insertion of a 'vector modification fragment'. This was prepared from the precursor of Os-amiR528 by eliminating the central miRNA-containing region while simultaneously creating an AfeI restriction site. The fragment was then introduced to the destination vector to produce a multipurpose 'Highly Efficient gene Silencing Compatible vector' (HESC vector). AfeI was used to produce linearized HESC vectors, and a blunt end PCR product that included amiRNA sequence was cloned into this site by a single ligation reaction to create the completed amiRNA vector. Tests showed that the method was highly efficient, and greatly reduced the time needed for vector construction and resulted in a DNA sequence identical to that of the current method, making it particularly suitable for use in a systems biology approach to functional genomic research.
- 相关文献
作者其他论文 更多>>
-
Microbial carbon and phosphorus metabolism regulated by C:N:P stoichiometry stimulates organic carbon accumulation in agricultural soils
作者:Wang, Xiangxiang;Zhang, Hongrui;Cao, Dan;Wei, Liang;Wang, Shuang;Ding, Jina;Chen, Jianping;Ge, Tida;Zhu, Zhenke;Wang, Xiangxiang;Zhang, Hongrui;Cao, Dan;Wu, Cuiyan;Wang, Xianting;Guo, Bin;Chen, Hao
关键词:Soil nutrients; Microbial metabolism; Ecoenzymatic stoichiometry; Microbial carbon use efficiency
-
Metabarcoding reveals response of rice rhizosphere bacterial community to rice bacterial leaf blight
作者:Jiang, Hubiao;Xu, Xinyan;Ogunyemi, Solabomi Olaitan;Ahmed, Temoor;Li, Bin;Fang, Yuan;Li, Xuqing;Yang, Yong;Yan, Chengqi;Chen, Jianping;Fang, Yuan;Chen, Jianping;Li, Bin
关键词:Bacterial leaf blight; Rice; 16S rRNA gene sequence; Rhizosphere; Microbial community
-
The peroxidase gene OsPrx114 activated by OsWRKY50 enhances drought tolerance through ROS scavenging in rice
作者:Zheng, Chao;Chen, Jianping;Zheng, Chao;Wang, Xuming;Xu, Yue;Liu, Xiuli;Cui, Weijun;Yan, Chengqi;Liu, Hongjia;Chen, Jianping;Zhou, Jie;Wu, Yueyan;Yan, Chengqi;Wang, Shaomin;Jiang, Xin;Lu, Yin
关键词:Drought tolerance; OsPrx114; OsWRKY50; Rice; ROS scavenging
-
Elevating plant immunity by translational regulation of a rice WRKY transcription factor
作者:Zheng, Chao;Zhou, Jie;Yuan, Xiaoya;Zheng, Ersong;Liu, Xiuli;Cui, Weijun;Chen, Jianping;Wang, Xuming;Zheng, Chao;Yuan, Xiaoya;Zheng, Ersong;Yan, Chengqi;Wu, Yueyan;Ruan, Wenyuan;Yi, Keke;Chen, Jianping
关键词:OsWRKY7; alternative translation; protein stability; basal immunity; Xoo
-
Nanobiotechnology to advance stress resilience in plants: Current opportunities and challenges
作者:Ijaz, Munazza;Ahmed, Temoor;Noman, Muhammad;Li, Bin;Khan, Fahad;Ahmed, Temoor;Zulfiqar, Faisal;Rizwan, Muhammad;Chen, Jianping;Siddique, Kadambot H. M.;Noman, Muhammad
关键词:Agriculture; Nanotechnology; Stress resilience; Nanosensors; Health risks
-
Adaption of tobacco rattle virus to its solanaceous hosts is related to the codon usage bias of the hosts and that of the viral 16 K gene
作者:Lin, Qi;Chen, Yi;Li, Miaomiao;Zheng, Hongying;Chen, Jianping;Yan, Fei;Lu, Yuwen;Zheng, Yuanting;Xu, Shengchun;Yin, Yueyan
关键词:Tobacco rattle virus (TRV); Codon usage bias; Host adaptation; 16K
-
OsTGAL1 suppresses the resistance of rice to bacterial blight disease by regulating the expression of salicylic acid glucosyltransferase OsSGT1
作者:Li, Dongyue;Zhou, Jie;Zheng, Chao;Zheng, Ersong;Liang, Weifang;Tan, Xiaojing;Xu, Rumeng;Yang, Yong;Liu, Xiuli;Chen, Jianping;Wang, Xuming;Li, Dongyue;Liang, Weifang;Chen, Jianping;Yan, Chengqi;Yi, Keke;Chen, Jianping
关键词:OsGRX17; OsSGT1; OsTGAL1; SA signaling; Xoo