Immune Protection Gap Between Porcine Reproductive and Respiratory Syndrome Subunit Vaccine (N Protein) and Live Vaccine
文献类型： 外文期刊
作者： Zhao, Mengpo 1 ; Zhang, Pian 1 ; Zhang, Xiaoxiao 1 ; Luo, Shengjun 1 ; Yuan, Ziguo 2 ; Huang, Yanju 1 ; Wang, Gang 1 ; Xiang, Hua 1 ; Huang, Yuan 1 ; Jin, Yuzhu 3 ; Chen, Jing 1 ; Wang, Xiaohu 1 ;
作者机构： 1.Inst Anim Hlth, Guangdong Acad Agr Sci, Guangdong Prov Key Lab Livestock Dis Prevent, Guangdong Prov Observat & Res Stn Anim Dis, Wushan St, Guangzhou 510640, Peoples R China
2.South China Agr Univ, Coll Vet Med, 483 Wushan Rd, Guangzhou 510642, Peoples R China
3.Jiaozuo City Prod Qual Inspect & Testing Ctr, Jiaozuo 454000, Peoples R China
关键词： porcine peproductive and pespiratory syndrome virus; subunit vaccine; live vaccine; immune protection
期刊名称：VACCINES （ 影响因子：3.4； 五年影响因子：3.7 ）
ISSN：
年卷期： 2025 年 13 卷 5 期
页码：
收录情况： SCI
摘要： Objectives: To evaluate the immunoprotective effect of a PRRSV N protein subunit vaccine on piglets using a live PRRSV vaccine as a control. Methods: The HEK-293T eukaryotic expression system was used to produce PRRSV N protein, and then PRRSV N protein was immunized with a commercial live PRRS vaccine. The immunoprotective effect of the PRRSV N protein subunit vaccine on piglets was evaluated by detecting the antibody level in the immunized piglets, and the clinical symptoms, pathological changes, and survival rate of the immunized piglets. Results: At 21 and 28 days after immunization, the serum N protein-specific antibody levels of piglets in the live PRRSV vaccine group were higher than those in the N protein group. After PRRSV infection, piglets in the N protein group and the DMEM group showed more severe clinical symptoms such as respiratory distress, loss of appetite, skin redness, and diarrhea than those in the live vaccine group. The rectal temperature of piglets in the live vaccine group remained below 40 degrees C, and only one piglet died on day 11 post-infection; in the PRRSV N protein group, the rectal temperature of some piglets exceeded 41 degrees C, and four piglets died on days 9, 11, 14, and 20 post-infection. In addition, pathologic damage to organs such as lungs, liver, lymph nodes, spleen, and kidneys was more severe in the N protein group than in the live vaccine group. Furthermore, histopathology and immunohistochemistry showed more pronounced organ damage (lungs, liver, lymph nodes, spleen, and kidneys) and higher viral loads in the N protein group compared to the live vaccine group. Conclusions: The PRRS subunit vaccine (N protein) expressed in the HEK-293T eukaryotic system did not protect piglets from heterologous PRRSV infection compared with the PRRS live vaccine.
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