Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development
文献类型: 外文期刊
作者: Huang, Quan-Sheng 1 ; Wang, Hai-Yun 1 ; Gao, Peng 1 ; Wang, Guo-Ying 2 ; Xia, Gui-Xian 1 ;
作者机构: 1.Chinese Acad Sci, Inst Microbiol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China
2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
3.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China
4.Xinjiang Acad Agr Sci, Urumqi 830094, Xinjiang, Peoples R China
期刊名称:PLANT CELL REPORTS ( 影响因子:4.57; 五年影响因子:4.463 )
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收录情况: SCI
摘要: The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals. In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins showed a subcellular distribution in the plasma membrane. In vitro assays indicated that GhCPK1 was a functional calcium-dependent kinase able to undergo autophosphorylation and phosphorylation of the known substrate histone III-S. Together, these results suggest that GhCPK1 may play a role in the calcium signaling events associated with fiber elongation.
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The phosphorylation of an actin depolymerizing factor by a calcium-dependent protein kinase regulates cotton fiber elongation
作者:Huang, Quan-Sheng;Chen, Xun-Ji;Li, Jian-Ping;Hao, Xiao-Yan;Chen, Guo;Zumuremu;Shao, Lin;Huang, Quan-Sheng;Chen, Xun-Ji;Li, Jian-Ping;Hao, Xiao-Yan;Chen, Guo;Zumuremu;Shao, Lin
关键词:Gossypium;Calcium-dependent protein kinase;Actin depolymerizing factor;Phosphorylation;Fiber elongation