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cDNA cloning, tissues, embryos and larvae expression analysis of Sox10 in half-smooth tongue-sole, Cynoglossus semilaevis

文献类型： 外文期刊

作者： Deng, Si-Ping 1 ; Chen, Song-Lin 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

3.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China

关键词： Cynoglossus semilaevis;Soxio;cDNA;Cloning;Expression

期刊名称：MARINE GENOMICS （ 影响因子：1.71； 五年影响因子：1.969 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A half-smooth tongue-sole, Cynoglossus semilaevis Sox10 (Accession no.: EU070763) was isolated from brain of tongue sole by using homologous cloning and RACE method. The complete cDNA of the tongue sole Sox10 contains a 35 bp 5'UTR, a 1338 bp open reading frame (ORF) encoding 445 amino acids and a 1155 bp 3'UTR. A condensed phylogenetic tree was constructed based on the amino acid sequences of tongue sole Sox10 and other well-defined vertebrate Sox. The overall topology of the tree showed the tongue sole Sox10 clusters with all Sox10. Alignment of amino acid residues of the tongue sole Sox10 gene with those from other vertebrate indicated high level conservation of amino acid sequence. The RT-PCR analysis demonstrated that the tongue sole Sox10 was highly expressed in brain, gills, skin and eyes, intermediately in spleen, heart, head-kidney and muscles, weakly expressed in kidneys and intestine and no expression in liver and gonad. The Sox10 was also expressed weakly in germ cell and zygote. We cannot detect the expression of the Sox10 in 8-cells stage. However it resumed expression weakly from blastula stage to middle of gastrula. And it expressed highly from neurula stage to 25 dah (day after hatching). It suggested that the Sox10 was involved in the development of embryos and larvae in tongue sole.

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