GhADT5 enhances alkali stress tolerance in cotton by regulating phenylalanine-derived flavonoid biosynthesis and antioxidant defense
文献类型： 外文期刊
作者： Wang, Lidong 1 ; Nan, Hongyu 3 ; Zhang, Menghao 1 ; Guang, Lijun 4 ; Meng, Junting 1 ; Liu, Mengyue 1 ; Meng, Yuan 1 ; Chen, Wenhua 1 ; Fan, Yapeng 1 ; Huang, Hui 1 ; Sun, Yuping 1 ; Yang, Zhining 1 ; Chen, Xiao 1 ; Wu, Fange 1 ; Song, Ruize 1 ; Wang, Shuai 1 ; Lu, Xuke 1 ; Chen, Xiugui 1 ; Zhao, Lanjie 1 ; Wang, Junjuan 1 ; Cui, Yupeng 1 ; Zhou, Xue-Rong 5 ; Wang, Ning 3 ; Feng, Keyun 3 ; Chen, Qin 2 ; Ye, Wuwei 1 ;
作者机构： 1.Chinese Acad Agr Sci, State Key Lab Cotton Biobreeding & Integrated Util, Natl Ctr Technol Innovat Comprehens Utilizat of Sa, Anyang Inst Technol,Inst Cotton Res,Res Base, Anyang 455000, Henan, Peoples R China
2.Xinjiang Agr Univ, Coll Agr, Engn Res Ctr Cotton, Minist Educ, 311 Nongda East Rd, Urumqi 830052, Xinjiang, Peoples R China
3.Gansu Acad Agr Sci, Inst Crop Sci, Lanzhou 730070, Gansu, Peoples R China
4.13th Div Xinjiang Prod & Construct Corps, Inst Agr Sci, Hami 839000, Xinjiang, Peoples R China
5.CSIRO Agr & Food, POB 1700, Canberra, ACT 2601, Australia
关键词：
Phenylalanine (phe); Flavonoid biosynthesis; Arogenate dehydratase (ADT);
期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）
ISSN： 1471-2229
年卷期： 2025 年 25 卷 1 期
页码：
收录情况： SCI
摘要： Phenylalanine (Phe), an aromatic amino acid, is a key precursor of flavonoids, which are crucial for plant growth and development. Arogenate dehydratase (ADT) catalyzes the final step in Phe biosynthesis. This study identified eleven ADT genes in G. hirsutum, twelve in G. barbadense, six in G. arboreum, and six in G. raimondii. Among them, GhADT5 exhibited the highest upregulation under alkali stress. Silencing GhADT5 using virus-induced gene silencing (VIGS) reduced cotton tolerance to alkali stress. GhADT5 silencing also led to decreased plant phenylalanine content, total flavonoid content, and activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). These reductions caused intracellular accumulation of Malondialdehyde (MDA) and reactive oxygen species (ROS). This oxidative damage ultimately reduced tolerance to alkali stress. In addition, silenced plants displayed reduced stomatal aperture, cellular deformation, and irregular intercellular breaks in the leaf epidermis. In summary, these findings suggest that GhADT5 may enhance resistance to alkali stress by regulating enzymatic and non-enzymatic antioxidant systems. This study highlights the role of GhADT5 under alkali stress and provides novel insights for breeding cotton varieties with improved stress tolerance.
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