Strain prioritization for lipopeptide-producing bacteria with antagonistic effects against Fusarium graminearum
文献类型： 外文期刊
作者： Wang, Gang 1 ; Wang, Yuting 1 ; Hu, Junqiang 4 ; Zhang, Xiaoyun 3 ; Chen, Daoming 1 ; Liu, Xin 1 ; Mohamed, Sherif Ramzy 5 ; Lee, Yin-Won 6 ; Yang, Chenye 7 ; Shi, Jianrong 1 ; Xu, Jianhong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Minist Agr & Rural Affairs, Inst Food Safety & Nutr, Collaborat Innovat Ctr Modern Grain Circulat & Saf, Nanjing 210014, Peoples R China
2.Jiangsu Ocean Univ, Sch Ocean Food & Biol Engn, Lianyungang 222005, Peoples R China
3.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
4.Nanjing Agr Univ, Coll Life Sci, Key Lab Agr Environm Microbiol, Minist Agr & Rural Affairs, Nanjing 210095, Peoples R China
5.Food Ind & Nutr Res Inst, Natl Res Ctr, Food Toxicol & Contaminants Dept, Giza 12411, Egypt
6.Seoul Natl Univ, Sch Agr Biotechnol, Seoul 08826, South Korea
7.Jiangsu Acad Agr Sci, Cent Lab, Nanjing 210014, Peoples R China
关键词： Strain prioritization; Biocontrol; Fusarium graminearum; lipopeptides; Bacillus amyloliquefaciens; antagonistic mechanism
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）
ISSN： 0882-4010
年卷期： 2025 年 206 卷
页码：
收录情况： SCI
摘要： The phytopathogenic fungus Fusarium graminearum causes severe plant disease such as wheat scab and poses threats to human health by mycotoxin contamination. Green agriculture's rise has brought growing attention to biological control methods, which contributes to pesticide reduction. In this study, we employed a quantitative reverse transcription PCR (qRT-PCR) based high-throughput screening method to identify bacterial strains that produce lipopeptides. By using this method, we identified Bacillus amyloliquefaciens strain 7D3, which exhibits high lipopeptide production and strong antifungal activity. The crude extract of 7D3 fermentation broth exhibited an EC50 of 75.09 +/- 6.72 mu g/mL. Analysis of the metabolites via liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) revealed numerous lipopeptides, such as surfactin and iturin. The strain inhibited Fusarium graminearum infection by disrupting the cell membrane, thus damaging the mycelial structure of the fungus and resulting in disordered hyphal osmotic characteristics and eventually hyphal rupture. This strain also inhibited sporulation as well as germination of Fusarium spores. Additionally, greenhouse trials demonstrated that the 7D3 strain effectively suppressed Fusarium infection in wheat while also inhibiting the accumulation of mycotoxins. This study provides an alternative method for screening biocontrol agents with activity against FHB.
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