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Differential proteomic analysis during the vegetative phase change and the floral transition in Malus domestica

文献类型： 外文期刊

作者： Zeng, Guang J. 2 ; Li, Chun M. 2 ; Zhang, Xin Z. 1 ; Han, Zhen H. 1 ; Yang, Feng Q. 2 ; Gao, Yan 1 ; Chen, Dong M. 2 ; Zh 1 ;

作者机构： 1.China Agr Univ, Inst Hort Plants, Beijing 100193, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli 066600, Peoples R China

3.Shenyang Agr Univ, Coll Hort, Shenyang 110161, Peoples R China

关键词： carbohydrate: metabolism;serine hydroxymethyltransferase: 9029-83-8;EC 2.1.2.1;floral transition

期刊名称：DEVELOPMENT GROWTH & DIFFERENTIATION （ 影响因子：2.053； 五年影响因子：2.124 ）

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收录情况： SCI

摘要： In order to identify the proteomic changes of apple (Malus domestica Borkh.) during the vegetative phase change and the floral transition, leaf protein of juvenile, adult vegetative and reproductive phase in a seedling ('Jonathan' x 'Golden Delicious') was extracted and analyzed by 2-D electrophoresis and Matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Seventy two gel spots with significant expression differences between ontogenetic phases were obtained. Five protein spots were only detected in leaves of juvenile phase and 11 were not; 17 spots were found exclusively in adult vegetative leaves; and only one spot solely appeared in reproductive leaves while 12 did not. Twenty six of the differentially expressed proteins identified were involved in photosynthesis. Seven enzymes were related to respiration and carbohydrate metabolism. Fifteen other proteins also presented qualitative or quantitative differences among developmental phases. The spatial distribution of one differentially expressed protein, serine hydroxymethyltransferase, was confirmed by enzyme linked immunosorbent assay and immunohistochemistry. These results strongly support the idea that the vegetative phase change and the floral transition are regulated independently during developmental process.

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[1]Protein phosphorylation differs significantly among ontogenetic phases in Malus seedlings. Wang, Yan,Wang, Yi,Han, Zhen Hai,Zhang, Xin Zhong,Zhao, Yong Bo,Chen, Dong Mei. 2014

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