您好，欢迎访问河北省农林科学院 机构知识库！
河北省农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Protein phosphorylation differs significantly among ontogenetic phases in Malus seedlings

文献类型： 外文期刊

作者： Wang, Yan 1 ; Wang, Yi 1 ; Zhao, Yong Bo 2 ; Chen, Dong Mei 2 ; Han, Zhen Hai 1 ; Zhang, Xin Zhong 1 ;

作者机构： 1.China Agr Univ, Inst Hort Plants, Beijing 100193, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli 066600, Peoples R China

关键词： Apple;Vegetative phase change;Floral transition;Phosphorylated proteomics

期刊名称：PROTEOME SCIENCE （ 影响因子：2.48； 五年影响因子：2.619 ）

ISSN： 1477-5956

年卷期： 2014 年 12 卷

页码：

收录情况： SCI

摘要： Background: Although protein phosphorylation is an important post-translational modification affecting protein function and metabolism, dynamic changes in this process during ontogenesis remain unexplored in woody angiosperms. Methods: Phosphorylated proteins from leaves of three apple seedlings at juvenile, adult vegetative and reproductive stages were extracted and subjected to alkaline phosphatase pre-treatment. After separating the proteins by two-dimensional gel electrophoresis and phosphoprotein-specific Pro-Q Diamond staining, differentially expressed phosphoproteins were identified by MALDI-TOF-TOF mass spectrometry. Results: A total of 107 phosphorylated protein spots on nine gels (three ontogenetic phases x three seedlings) were identified by MALDI-TOF-TOF mass spectrometry. The 55 spots of ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) large-chain fragments varied significantly in protein abundance and degree of phosphorylation among ontogenetic phases. Abundances of the 27 spots corresponding to Rubisco activase declined between juvenile and reproductive phases. More extensively, phosphorylated beta-tubulin chain spots with lower isoelectric points were most abundant during juvenile and adult vegetative phases. Conclusions: Protein phosphorylation varied significantly during vegetative phase change and floral transition in apple seedlings. Most of the observed changes were consistent among seedlings and between hybrid populations.

  • 相关文献

[1]Differential proteomic analysis during the vegetative phase change and the floral transition in Malus domestica. Zhang, Xin Z.,Han, Zhen H.,Gao, Yan,Wang, Yi,Zeng, Guang J.,Li, Chun M.,Yang, Feng Q.,Chen, Dong M.,Zhao, Yong B.,Zeng, Guang J.,Teng, Yun L.,Dong, Wen X..

[2]Differential expression and modification of proteins during ontogenesis in Malus domestica. Cao, Xin,Gao, Yan,Wang, Yi,Han, Zhen H.,Zhang, Xin Z.,Li, Chun M.,Zhao, Yong B.. 2011

[3]A dense SNP genetic map constructed using restriction site-associated DNA sequencing enables detection of QTLs controlling apple fruit quality. Sun, Rui,Chang, Yuansheng,Wang, Yi,Li, Hui,Wu, Ting,Zhang, Xinzhong,Han, Zhenhai,Yang, Fengqiu,Zhao, Yongbo,Chen, Dongmei. 2015

[4]Dynamics of Endogenous Cytokinins during Phase Change in Malus domestica Borkh. Zhang, Xin Zhong,Zhao, Yong Bo,Wang, Guang Peng,Chang, Rui Feng,Li, Chun Min,Zhang, Xin Zhong,Shu, Huai Rui,Zhang, Xin Zhong. 2008

[5]Inheritance of and Molecular Markers for Susceptibility of Malus domestica to Fruit Ring Rot (Botryosphaeria dothidea). Zhuang, Yan,Liu, Hai Tao,Wang, Yi,Han, Zhen Hai,Zhang, Xin Zhong,Li, Chun Min,Zhao, Yong Bo,Chen, Dong Mei. 2011

[6]Genetic Mapping Reveals Sophisticated Responses of Malus domestica to Botryosphaeria dothidea Isolates. Cui, Mei Sha,Yang, Li Li,Han, Yuan Yuan,Wang, Yi,Zhang, Xin Zhong,Han, Zhen Hai,Zhang, Qiong,Han, Yue Peng,Zhao, Yong Bo,Li, Chun Min,Chen, Dong Mei,Yang, Feng Qiu.

[7]Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 1999

[8]Cryopreservation of apple in vitro shoot tips by the droplet freezing method. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Chen, SY. 1999

[9]Cell wall metabolism and related gene expression in Malus domestica Borkh. during fruit growth and softening. Qi, Xiu-dong,Wei, Jian-mei,Wei, Jian-mei,Li, Haishan,Zhao, Dan.

[10]Diversity of flesh polyphenols and their progressive dilution during fruit expansion in Malus germplasm. Zhao, Jirong,Cao, Ke,Shen, Fei,Wu, Ting,Wang, Yi,Han, Zhenhai,Zhang, Xinzhong,Liu, Guojian,Chang, Ruifeng.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河北省农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台