Molecular Characterization of Burkholderia cepacia Complex Isolates Causing Bacterial Fruit Rot of Apricot
文献类型： 外文期刊
作者： Li, Bin 1 ; Fang, Yuan 1 ; Zhang, Guoqing 1 ; Yu, Rongrong 2 ; Lou, Miaomiao 1 ; Xie, Guanlin 1 ; Wang, Yanli 3 ; Sun, Gu 1 ;
作者机构： 1.Zhejiang Univ, State Key Lab Rice Biol, Key Lab Mol Biol Crop Pathogens & Insects, Minist Agr,Inst Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China
2.Zhejiang Univ Technol, Hangzhou 310032, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China
4.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinh
关键词： recA-HaeIII
期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.795； 五年影响因子：2.314 ）
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年卷期：
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收录情况： SCI
摘要： The Burkholderia cepacia complex isolates causing bacterial fruit rot of apricot were characterized by speciesspecific PCR tests, recA-HaeIII restriction fragment length polymorphism (RFLP) assays, rep-PCR genomic fingerprinting, recA gene sequencing, and multilocus sequence typing (MLST) analysis. Results indicated that the isolates Bca 0901 and Bca 0902 gave positive amplifications with primers specific for B. vietnamiensis while the two bacterial isolates showed different recA-RFLP and rep-PCR profiles from those of B. vietnamiensis strains. In addition, the two bacterial isolates had a higher proteolytic activity compared with that of the non-pathogenic B. vietnamiensis strains while no cblA and esmR marker genes were detected for the two bacterial isolates and B. vietnamiensis strains. The two bacterial isolates were identified as Burkholderia seminalis based on recA gene sequence analysis and MLST analysis. Overall, this is the first characterization of B. seminalis that cause bacterial fruit rot of apricot.
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