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Functional Characterization of the Ryanodine Receptor Gene in Diaphorina citri

文献类型： 外文期刊

作者： Liu, Tian-Sheng 1 ; Sun, Xue-Li 1 ; Bin, Min-Liang 1 ; Yi, Gan-Jun 1 ; Zhang, Xin-Xin 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou 510640, Peoples R China

2.South China Agr Univ, Coll Life Sci, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Peoples R China

关键词： ryanodine receptors; Diaphorina citri; mRNA expression; alternative splicing

期刊名称：LIFE-BASEL （ 影响因子：3.251； ）

ISSN：

年卷期： 2022 年 12 卷 12 期

页码：

收录情况： SCI

摘要： The Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae) is a major citrus pest spread around the world. It is also a vector of the bacterium 'Candidatus Liberibacter asiaticus', considered the cause of the fatal citrus disease huanglongbing (HLB). Insect ryanodine receptors (RyRs) are the primary target sites of diamide insecticides. In this study, full-length RyR cDNA from D. citri (named DcRyR) was isolated and identified. The 15,393 bp long open reading frame of DcRyR encoded a 5130 amino acid protein with a calculated molecular weight of 580,830 kDa. This protein had a high sequence identity (76-79%) with other insect homologs and a low sequence identity (43-46%) with mammals. An MIR domain, two RIH domains, three SPRY domains, four RyR repeat domains, an RIH-associated domain at the N-terminus, two consensus calcium-binding EF-hands, and six transmembrane domains were among the characteristics that DcRyR shared with insect and vertebrate RyRs. In expression analysis, the DcRyR gene displayed transcript abundance in all tissues and developmental stages as well as gene-differential and stage-specific patterns. In addition, diagnostic PCR experiments revealed that DcRyR had three potential alternative splice variants and that splicing events might have contributed to the various functions of DcRyR. However, diamide resistance-related amino acid residue mutations I4790M/K and G4946E were not found in DcRyR. These results can serve as the basis for further investigation into the target-based diamide pesticide resistance of D. citri.

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