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Solvent gradient elution for comprehensive separation of constituents with wide range of polarity in Apocynum venetum leaves by high-speed counter-current chromatography

文献类型： 外文期刊

作者： Zhang, Yuchi 1 ; Liu, Chunming 1 ; Zhang, Zhengkun 2 ; Qi, Yanjuan 1 ; Wu, Guimei 1 ; Li, Sainan 1 ;

作者机构： 1.Changchun Normal Univ, Cent Lab, Changchun 130032, Peoples R China

2.Jilin Acad Agr Sci, Inst Plant Protect, Gongzhuling, Peoples R China

关键词： Apocynum venetum L.;Gradient elution mode;High-speed counter-current chromatography;Separation and purification

期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）

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年卷期：

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收录情况： SCI

摘要： A novel gradient elution was efficiently utilized for the separation of the chemical components with a wide range of polarity from the mixed extract of the Chinese medicinal herb Apocynum venetum or mixed standards by high-speed counter-current chromatography. Three sets of solvent systems, n-hexane-ethyl acetate-methanol-water (1.5:3.5:2:4.5 v/v/v/v), ethyl acetate-methanol-water (5:2:5 v/v/v) and n-butanol-methanol-water (5:1:5 v/v/v) were used for the one-step elution. The separation was initiated by filling the column with the lower phase of n-hexane-ethyl acetate-methanol-water (1.5:3.5:2:4.5 v/v/v/v) as a stationary phase followed by elution with the upper phase of n-hexane-ethyl acetate-methanol-water (1.5:3.5:2:4.5 v/v/v/v) to separate the hydrophobic compounds (tail to head). Then the mobile phase was switched to the upper phase of ethyl acetate-methanol-water (5:2:5 v/v/v) to elute the moderate hydrophobic compounds, and finally the hydrophilic compounds still retained in the column were fractionated by eluting the column with the upper phase of n-butanol-methanol-water (5:1:5 v/v/v). A total of 13 compounds including adhyperforin, hyperforin, amentoflavone, biapigenin, quercetin, astragalin, trifolin, isoquercetin, hyperside, acetyled hyperside, rutin, chlorogenic acid and quercetin-3-O-β-D-glucosyl-β-D-glucopyranoside were successfully separated via the three sets of solvent systems in one-step operation for 90 min.

  • 相关文献

[1]Comprehensive separation and identification of chemical constituents from Apocynum venetum leaves by high-performance counter-current chromatography and high performance liquid chromatography coupled with mass spectrometry. Zhang, Yuchi,Liu, Chunming,Wang, Jing,Wu, Guimei,Li, Sainan,Zhang, Zhengkun.

[2]Fast differentiation of Apocynum venetum with related species by UPLC/MS and UPLC/SPE/NMR. Zhang, Yuchi,Liu, Chunming,Qi, Yanjuan,Wu, Guimei,Li, Sainan,Zhang, Zhengkun.

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