Screening and identification of a highly lipolytic bacterial strain from barbecue sites in Hainan and characterization of its lipase
文献类型： 外文期刊
作者： Ma, Qiongli 1 ; Sun, Xuepiao 1 ; Gong, Shufeng 1 ; Zhang, Jiaming 1 ;
作者机构： 1.CATAS, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
2.CATAS, Spice & Beverage Res Inst, Xinglong 571533, Hainan, Peoples R China
3.Hainan Univ, Coll Agr, Haikou 571101, Hainan, Peoples R China
关键词： Burkholderia cepacia;Ferric uptake regulator;Lipase;Lipolytic bacteria;Plate screening
期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：2.112； 五年影响因子：2.151 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： A cheap and rapid screening method for isolation of lipolytic bacteria was established. A total of 145 lipolytic strains were isolated from oil-contaminated soil samples at barbeque sites in Haikou, China. Highly lipolytic strains were screened based on the formation of clearance zones on turbid solid media supplemented with emulsified peanut oil. One strain, C737-11, had the highest lipolytic activity and was further analyzed. This strain had physiological and biochemical characteristics that were similar to the genus Burkholderia. Phylogenetic analysis based on the ferric uptake regulator (fur) gene sequences located the strain to the clade of Burkholderia cepacia, Genomovar I, while resolution of the phylogeny based on 16S rRNA sequences was not good enough to distinguish Burkholderia species. The fermentation conditions were optimized and the optimal medium inducing or supporting lipase production was 0.5% glucose, 2% peanut oil, 2% peptone, 0.1% (NH4)(2)SO4, 0.1% K2HPO4 center dot 3H(2)O and 0.05% MgSO4 center dot 7H(2)O at pH 8.0. The optimal fermentation temperature was 37 degrees C, and the best fermentation time was 72 h. Under these optimal conditions, lipolytic activity reached 10.5 U mL(-1). The lipase produced by C737-11 was thermally stable and had an optimal reaction pH of 8.0 and an optimal reaction temperature of 37 degrees C.
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