文献类型： 外文期刊
作者： Bai, Yalong 1 ; Pu, Chunmin 1 ; Suo, Yujuan 1 ; Zhang, Xufu 3 ; Qu, Yang 1 ; Feng, Bo 1 ; Huang, Liujuan 1 ; Shao, Yi 1 ; Dai, Yingchun 4 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Shanghai, Peoples R China
2.Univ South China, Coll Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang, Hunan, Peoples R China
3.Southern Med Univ, Sch Tradit Chinese Med, Guangzhou, Guangdong, Peoples R China
4.Southern Med Univ, Sch Publ Hlth & Trop Med, Dept Epidemiol, Guangdong Prov Key Lab Trop Dis Res, Guangzhou, Guangdong, Peoples R China
5.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
关键词： human norovirus; detection; magnetic capture; RT-qPCR; strawberry
期刊名称：FOOD FRONTIERS （ 影响因子：9.9； 五年影响因子：10.0 ）
ISSN：
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Human norovirus is a leading cause of foodborne illness worldwide, and its detection in foods remains a challenge due to the difficulty of eluting and enriching trace amounts of viral particles. In this study, we developed porcine gastric mucin-labeled magnetic capture probes and optimized the conditions for their binding with norovirus, elution of viral particles, and RNA isolation based on the previous research. We evaluated the magnetic capture-based pretreatment method in artificially contaminated strawberry samples and compared it with the conventional polyethylene glycol precipitation-based method using reverse transcriptional-quantitative PCR (RT-qPCR). The results revealed that Tris center dot HCl (pH 9.5) was the optimal buffer for the binding of magnetic probes with viral particles, and RNA recovery rate obtained by heating-based release was significantly higher than that obtained by a commercial kit, with only active virus being captured. This buffer was similar to the RT-qPCR buffer, which facilitated the sensitivity of RT-qPCR. Furthermore, the buffer was an effective solution for eluting viral particles from foods in previous research and was optimized to ensure maximum elution and no inhibitory effect on viral capture. We explored multiple factors or steps to ensure that the sensitivity of our method was three orders of magnitude higher than that of the conventional method. Our findings suggest that magnetic capture-based detection has great potential for the sensitive detection of human norovirus.
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