The bHLH transcription factor AcB2 regulates anthocyanin biosynthesis in onion (Allium cepa L.)
文献类型： 外文期刊
作者： Li, Xiaojie 1 ; Cao, Linjiao 1 ; Jiao, Bangbang 1 ; Yang, Haifeng 4 ; Ma, Changsheng 5 ; Liang, Yi 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China
3.Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China
4.Lianyungang Acad Agr Sci, Vegetable Res Ctr, Yingbin Blvd, Lianyungang 222000, Jiangsu, Peoples R China
5.Henan Agr Univ, Coll Hort, Dept Vegetables, Zhengzhou 450046, Henan, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 2021影响因子：7.291； 五年影响因子：7.487 ）
ISSN： 2662-6810
年卷期： 2022 年 9 卷
收录情况： SCI
摘要： Onion bulb color is a key breeding trait. The red bulb color is caused by the presence of anthocyanins, which are products of the flavonoid synthesis pathway. Research on flavonoid regulation in onion is lagging compared with that in other crops. AcB2 encodes a basic helix-loop-helix (bHLH) transcription factor, and its transcription is positively associated with anthocyanin accumulation and correlated with the expression of AcMYB1, which is an activator in the flavonoid biosynthetic pathway in onion. Phylogenetic analysis showed that AcB2 was grouped into the TRANSPARENT TESTA 8 (TT8) Glade of the bHLH IIIf subgroup. The AcB2 protein contained an MYB-interacting region and physically interacted with AcMYB1 in yeast and tobacco leaves. AcMYB1 directly bound to the promoters of anthocyanidin synthase (AcANS) and flavonoid 3-hydroxylase 1 (AcF3H1) and activated their expression. The coexpression of AcB2 with AcMYB1 in Arabidopsis thaliana protoplasts dramatically increased the expression of AcANS and AcF3H1 compared with that under the expression of AcMYB1 alone. Transient co-overexpression of AcB2 with AcMYB1 induced anthocyanin accumulation in the epithelial cells of onion bulbs. Complementation of the Arabidopsis tt8-1 mutant with AcB2 restored pigmentation defects in tt8-1. In addition, AcB2 physically interacted with AtTT2 in yeast cells and tobacco leaves, indicating that the functions of AcB2 were similar to those of AtTT8. Together, these results demonstrated that AcB2 enhanced the function of AcMYB1 in upregulating anthocyanin biosynthesis in onion, which provides a theoretical basis for breeding onions with higher anthocyanin contents.
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