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ALKBH5 regulates chicken adipogenesis by mediating LCAT mRNA stability depending on m6A modification

文献类型： 外文期刊

作者： Chao, Xiaohuan 1 ; Guo, Lijin 1 ; Ye, Chutian 1 ; Liu, Aijun 1 ; Wang, Xiaomeng 1 ; Ye, Mao 1 ; Fan, Zhexia 1 ; Luan, Kang 1 ; Chen, Jiahao 3 ; Zhang, Chunlei 3 ; Liu, Manqing 1 ; Zhou, Bo 3 ; Zhang, Xiquan 1 ; Li, Zhenhui 1 ; Luo, Qingbin 1 ;

作者机构： 1.South China Agr Univ, State Key Lab Livestock & Poultry Breeding, Guangzhou, Peoples R China

2.South China Agr Univ, Coll Anim Sci, Guangzhou, Peoples R China

3.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Peoples R China

关键词： MeRIP-seq; Chicken; Adipogenesis; ALKBH5; LCAT; Stability

期刊名称：BMC GENOMICS （ 影响因子：3.5； 五年影响因子：4.1 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Background Previous studies have demonstrated the role of N6-methyladenosine (m6A) RNA methylation in various biological processes, our research is the first to elucidate its specific impact on LCAT mRNA stability and adipogenesis in poultry. Results The 6 100-day-old female chickens were categorized into high (n = 3) and low-fat chickens (n = 3) based on their abdominal fat ratios, and their abdominal fat tissues were processed for MeRIP-seq and RNA-seq. An integrated analysis of MeRIP-seq and RNA-seq omics data revealed 16 differentially expressed genes associated with to differential m(6)A modifications. Among them, ELOVL fatty acid elongase 2 (ELOVL2), pyruvate dehydrogenase kinase 4 (PDK4), fatty acid binding protein 9 (PMP2), fatty acid binding protein 1 (FABP1), lysosomal associated membrane protein 3 (LAMP3), lecithin-cholesterol acyltransferase (LCAT) and solute carrier family 2 member 1 (SLC2A1) have ever been reported to be associated with adipogenesis. Interestingly, LCAT was down-regulated and expressed along with decreased levels of mRNA methylation methylation in the low-fat group. Mechanistically, the highly expressed ALKBH5 gene regulates LCAT RNA demethylation and affects LCAT mRNA stability. In addition, LCAT inhibits preadipocyte proliferation and promotes preadipocyte differentiation, and plays a key role in adipogenesis. Conclusions In conclusion, ALKBH5 mediates RNA stability of LCAT through demethylation and affects chicken adipogenesis. This study provides a theoretical basis for further understanding of RNA methylation regulation in chicken adipogenesis.

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