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SmKFB5 protein regulates phenolic acid biosynthesis by controlling the degradation of phenylalanine ammonia-lyase in Salvia miltiorrhiza

文献类型： 外文期刊

作者： Yu, Haizheng 1 ; Li, Dongyue 2 ; Yang, Dongfeng 1 ; Xue, Zheyong 5 ; Li, Jie 6 ; Xing, Bingcong 1 ; Yan, Kaijing 7 ; Han, 1 ;

作者机构： 1.Zhejiang Sci Tech Univ, Coll Life Sci & Med, Zhejiang Prov Key Lab Plant Secondary Metab & Reg, Hangzhou 310018, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.Chinese Acad Sci & Minist Water Resource, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China

4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

5.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China

6.John Innes Ctr, Dept Metab Biol, Norwich NR4 7UH, Norfolk, England

7.Tasly Holding Grp Co Ltd, Tasly R&D Inst, Tianjin 300410, Peoples R China

关键词： KFB; phenolic acids; phenylalanine ammonia-Iyase; post-translational regulation; Salvia miltiorrhiza; ubiqitin-26S proteasome

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2021 年 72 卷 13 期

页码：

收录情况： SCI

摘要： Phenolic acids are the major secondary metabolites and significant bioactive constituents of the medicinal plant Salvia miltiorrhiza. Many enzyme-encoding genes and transcription factors involved in the biosynthesis of phenolic acids have been identified, but the underlying post-translational regulatory mechanisms are poorly understood. Here, we demonstrate that the S. miltiorrhiza Kelch repeat F-box protein SmKFB5 physically interacts with three phenylalanine ammonia-Iyase (PAL) isozymes and mediates their proteolytic turnover via the ubiquitin-26S proteasome pathway. Disturbing the expression of SmKFB5 reciprocally affected the abundance of SmPAL protein and the accumulation of phenolic acids, suggesting that SmKFB5 is a post-translational regulator responsible for the turnover of PAL and negatively controlling phenolic acids. Furthermore, we discovered that treatment of the hairy root of S. miltiorrhiza with methyl jasmonate suppressed the expression of SmKFB5 while inducing the transcription of SmPAL1 and SmPAL3. These data suggested that methyl jasmonate consolidated both transcriptional and post-translational regulation mechanisms to enhance phenolic acid biosynthesis. Taken together, our results provide insights into the molecular mechanisms by which SmKFB5 mediates the regulation of phenolic acid biosynthesis by jasmonic acid, and suggest valuable targets for plant breeders in tailoring new cultivars.

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