您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae

文献类型： 外文期刊

作者： Wang, Zhi-Peng 1 ; Zhang, Xin-Yue 1 ; Ma, Yan 1 ; Ye, Jing-Run 1 ; Jiang, Jing 2 ; Wang, Hai-Ying 3 ; Chen, Wei 1 ;

作者机构： 1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China

2.Suzhou Univ Sci & Technol, Sch Environm Sci & Engn, Suzhou 215009, Jiangsu, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Sustainable Dev Polar Fishery, Qingdao 266071, Peoples R China

关键词： Lipid; Soy molasses; Whey powder; alpha-Galactosidase; beta-Galactosidase

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN：

年卷期： 2021 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Background: Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. Results: In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C-16:0, C-18:0, C-18:1, and C-18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three alpha-galactosidases and five beta-galactosidases in the strain were cloned and analyzed. Changes of transcriptional levels indicated GalB and GalC were key alpha-galactosidases, and GalG was key beta-galactosidase. In 10 L fermentor, lipid production from SM and WP achieved 6.45 g/L and 6.13 g/L, respectively. beta-galactosidase was responsible for lactose hydrolysis; sucrase and alpha-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. Conclusions: This is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess. Graphic abstract

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台