A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops
文献类型： 外文期刊
作者： Yang, Qianwen 1 ; Wang, Yu 1 ; Liu, Xiaofeng 2 ; Liu, Hua 1 ; Bao, Huifang 4 ; Wang, Jinbin 1 ; Zeng, Haijuan 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai, Peoples R China
2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China
3.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspect & Supervis Ctr, Shanghai, Peoples R China
4.Xinjiang Acad Agr Sci, Inst Appl Microbiol, Urumqi, Peoples R China
关键词： immunosensor; thionine; gold nanoparticles; label free; genetically modified crops
期刊名称：FRONTIERS IN CHEMISTRY （ 影响因子：5.545； 五年影响因子：5.869 ）
ISSN： 2296-2646
年卷期： 2021 年 9 卷
页码：
收录情况： SCI
摘要： Genetically modified (GM) crops containing phosphinothricin acetyltransferase (PAT) protein has been widely planted worldwide. The development of a rapid method for detecting PAT protein is of great importance to food supervision. In this study, a simple label-free electrochemical immunosensor for the ultrasensitive detection of PAT protein was constructed using thionine (Thi)/gold nanoparticles (AuNPs) as signal amplification molecules and electrochemically active substances. Under optimum conditions, the limits of detection of the sensor for soybean A2704-12 and maize BT-176 were 0.02% and 0.03%, respectively. The sensor could detect crops containing PAT protein and had no cross-reaction with other proteins. After storage at 4 degrees C for 33 days, the sensor still retained 82.5% of the original signal, with a relative standard deviation (RSD) of 0.92%. The recoveries of the sensor for soybean A2704-12 and maize BT-176 were 85%-108% and 98%-113%, respectively. The developed PAT-target immunosensor with high sensitivity, specificity, and satisfactory reproducibility and accuracy will be a useful tool in the trace screening of GM crops. Moreover, this design concept can be extended to other proteins by simply changing the antibody.
- 相关文献
作者其他论文 更多>>
-
Optimization of the fermentation media and growth conditions of Bacillus velezensis BHZ-29 using a Plackett-Burman design experiment combined with response surface methodology
作者：Shi, YingWu;Yang, HongMei;Chu, Ming;Wang, Ning;Bao, HuiFang;Zhan, FaQiang;Yang, Rong;Lou, Kai;Shi, YingWu;Yang, HongMei;Chu, Ming;Wang, Ning;Bao, HuiFang;Zhan, FaQiang;Yang, Rong;Lou, Kai;Shi, YingWu;Niu, XinXiang;Yang, HongMei;Chu, Ming;Wang, Ning;Niu, XinXiang
关键词：optimization; fermentation media; fermentation conditions; Bacillus velezensis BHZ-29; Plackett-Burman design; response surface methodology
-
Comparative Transcriptome and Endophytic Bacterial Community Analysis of Morchella conica SH
作者：Lu, Bei B.;Wu, Guo G.;Sun, Yu;Wu, Xiao;Jiang, Wei;Li, Peng;Huang, Yan N.;Wang, Jin B.;Liu, Hua;Song, Li L.;Mo, Qin;Pan, Ai H.;Tang, Xue M.;Zhang, Liang S.;Zhao, Yong C.;Yang, Yan;Long, Xuan Q.;Cui, Wei D.;Zhang, Chao;Zhang, Chao;Wang, Xu;Wang, Xu
关键词：Morchella conica SH; genomic; transcriptomic; fruiting body; endophytic bacterial communities
-
Rapid detection of P-35S and T-nos in genetically modified organisms by recombinase polymerase amplification combined with a lateral flow strip
作者：Liu, Hua;Wang, Jinbin;Bai, Lan;Pan, Aihu;Tang, Xueming;Liu, Hua;Wang, Jinbin;Bai, Lan;Pan, Aihu;Tang, Xueming;Liu, Hua;Wang, Jinbin;Li, Peng;Bai, Lan;Jia, Junwei;Tang, Xueming;Long, Xuanqi;Cui, Weidong
关键词：GMOs; RPA-LFD; P-35S; T-nos; Field application
-
The chemical nature of soil phosphorus in response to long-term fertilization practices: Implications for sustainable phosphorus management
作者：Liu, Jin;Han, Chaoqun;Sui, Peng;Chen, Yuanquan;Zhao, Yuhang;Yang, Jianjun;Cade-Menun, Barbara J.;Hu, Yongfeng;Li, Jumei;Liu, Hua;Ma, Yibing
关键词：Soil; Manure; Molecularspeciation; SolutionP-NMR; P-XANES; Newberyite
-
Comparative characterization of extracellular enzymes secreted by Phanerochaete chrysosporium during solid-state and submerged fermentation
作者：Liu, Jiawen;Yang, Jinshui;Wang, Ruonan;Liu, Liang;Zhang, Yu;Jang, Jin Myong;Yuan, Hongli;Bao, Huifang;Wang, Entao
关键词：Phanerochaete chrysosporium; Lignocellulose; Cellulase; Hemicellulase; Secretome; Carbohydrate-binding module
-
Degradation of pendimethalin by the yeast YC2 and determination of its two main metabolites
作者：Han, Yajie;Ye, Bang-Ce;Dai, Bin;Han, Yajie;Tang, Zonggui;Bao, Huifang;Wu, Dongmei;Deng, Xiaolin;Guo, Gaowei
关键词：
-
Characterization of a high performance alpha-galactosidase from Irpex lacteus and its usage in removal of raffinose family oligosaccharides from soymilk
作者：Jang, Jin Myong;Yang, Yi;Wang, Ruonan;Yuan, Hongli;Yang, Jinshui;Jang, Jin Myong;Bao, Huifang
关键词：alpha-Galactosidase; Irpex lacteus; Raffinose family oligosaccharides; Soymilk; Pichia pastoris
