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Engineering photo-methylotrophic Methylobacterium for enhanced 3-hydroxypropionic acid production during non-growth stage fermentation

文献类型： 外文期刊

作者： Ma, Zeng-Xin 1 ; Feng, Chen-Xi 1 ; Song, Ya-Zhen 1 ; Sun, Jing 1 ; Shao, Yi 1 ; Song, Shu-Zhen 1 ; Wan, Bin 1 ; Zhang, Cong 1 ; Fan, Huan 3 ; Bao, Kai 4 ; Yang, Song 1 ;

作者机构： 1.Qingdao Agr Univ, Sch Life Sci, Shandong Prov Key Lab Appl Mycol, Qingdao 266109, Shandong, Peoples R China

2.Qingdao Agr Univ, Qingdao Int Ctr Microbes Utilizing Biogas, Qingdao 266109, Shandong, Peoples R China

3.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Engn Res Ctr Anim Hlth Farming, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China

4.Hubei Univ, Sch Life Sci, Wuhan 430062, Hubei, Peoples R China

5.Tianjin Univ, Key Lab Syst Bioengn, Minist Educ, Tianjin 300072, Peoples R China

关键词： Methanol; Photo-methylotrophy; Two-stage fermentation; Methylobacterium extorquens; Energy- and reducing equivalent-consuming chemicals

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2024 年 393 卷

页码：

收录情况： SCI

摘要： This study explored the potential of methanol as a sustainable feedstock for biomanufacturing, focusing on Methylobacterium extorquens, a well-established representative of methylotrophic cell factories. Despite this bacterium's long history, its untapped photosynthetic capabilities for production enhancement have remained unreported. Using genome-scale flux balance analysis, it was hypothesized that introducing photon fluxes could boost the yield of 3-hydroxypropionic acid (3-HP), an energy- and reducing equivalent-consuming chemicals. To realize this, M. extorquens was genetically modified by eliminating the negative regulator of photosynthesis, leading to improved ATP levels and metabolic activity in non-growth cells during a two-stage fermentation process. This modification resulted in a remarkable 3.0-fold increase in 3-HP titer and a 2.1-fold increase in its yield during stage (II). Transcriptomics revealed that enhanced light-driven methanol oxidation, NADH transhydrogenation, ATP generation, and fatty acid degradation were key factors. This development of photomethylotrophy as a platform technology introduced novel opportunities for future production enhancements.

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