Leptins regulate the migration, proliferation, apoptosis, and synthesis of sexual steroid in tongue sole (Cynoglossus semilaevis) ovarian cells
文献类型： 外文期刊
作者： Cai, Xin 1 ; Meng, Zhaojun 1 ; Xu, Yongjiang 1 ; Jiang, Yan 1 ; Cui, Aijun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Joint Lab Deep Blue Fishery Engn, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China
关键词： Tongue sole leptin; Knock down; Estradiol hormone
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2024 年 278 卷
页码：
收录情况： SCI
摘要： Leptin is an important hormone in mammals, which plays a key role in regulating reproduction and energy metabolism. However, there are few studies on the function of leptin in reproductive regulation in fish, especially on tongue sole (Cynoglossus semilaevis). Thus, in this study, we firstly exploited the basic function of tongue sole leptins, the migration and growth rate of ovarian cells were reduced after knocking down lepA and lepB in ovarian cells, while increasing the apoptosis rate. Then both rlepA and rlepB were proved to be combined with lepR to further exert functions by dual luciferase assay. Transcriptome sequencing showed that differentially expressed genes (DEGs) were mainly enriched in KEGG pathways related to membrane receptors, fatty acid synthesis, growth, etc. when lepA and lepB were knocked down or additionally added in vitro. Additionally, the estradiol (E2) hormone was increased significantly after knocking down lepB. Finally, based on DEGs and the signaling pathways they participated in, we proposed a hypothesis about the signaling pathways in which leptin may be involved in ovarian cells. Taken together, these results provide new insights into the role of leptin in the regulation of physiological functions such as ovarian growth and development.
- 相关文献
作者其他论文 更多>>
-
Effects of leptin on fatty acid metabolism in the liver and reproductive function of tongue sole (Cynoglossus semilaevis) in vivo
作者：Cai, Xin;Li, Yuting;Meng, Zhaojun;Cui, Aijun;Jiang, Yan;Xu, Yongjiang;Meng, Zhaojun
关键词：Leptin; Tongue sole; Lipid metabolism; Regulatory mechanism; Reproductive function
-
The effects of 5-Hydroxymethylfurfural in mitigating transportation stress of large yellow croaker (Larimichthys crocea)
作者：Zheng, Jichang;Feng, Yuan;Xu, Yongjiang
关键词：Larimichthys crocea; 5-Hydroxymethylfurfural; Transportation stress; PI3K-AKT signaling pathway; Heat shock protein 90 alpha
-
Heavy metal distribution and ecological risk in surface sediments of the Bohai Sea
作者：Li, Shilin;Zhang, Jianshe;Li, Shilin;Chen, Jianlei;Zhang, Xuzhi;Xu, Yongjiang;Xu, Yong;Li, Shilin;Chen, Jianlei;Zhang, Xuzhi;Xu, Yongjiang;Xu, Yong
关键词：
-
Yellowtail kingfish Seriola aureovittata farmed in offshore net cage response to high density by regulating protein and glucose metabolism
作者：Jiang, Yan;Xu, Yongjiang;Cui, Aijun;Wang, Kaijie;Wang, Bin;Yu, Chaoyong;Xue, Zhiyong
关键词：
Seriola aureovittata ; Stocking density; Crowding stress; Growth performance; Physiological parameter -
Neurokinin B in the yellowtail kingfish (Seriola lalandi) and its potential role in controlling reproduction
作者：Tian, Zhenfang;Yu, Zhihua;Xu, Yongjiang;Cui, Aijun;Jiang, Yan;Wang, Bin;Tian, Zhenfang;Xu, Yongjiang;Cui, Aijun;Jiang, Yan;Wang, Bin;Yu, Zhihua
关键词：tac3; NKB; NKF; Reproduction; Yellowtail kingfish
-
Characterization of adaptive expression regulation of yellowtail kingfish (Seriola lalandi) leptin, receptor, and receptor overlapping transcript genes in response to fasting and re-feeding strategies
作者：Cai, Xin;Li, Ying;Cui, Aijun;Jiang, Yan;Wang, Bin;Meng, Zhaojun;Xu, Yongjiang
关键词：Yellowtail kingfish; Leptin; Receptor; Leptin receptor overlapping transcript; Fasting and re-feeding
-
Cloning, phylogenetic and expression analysis of two MyoDs in yellowtail kingfish (Seriola lalandi)
作者：Shi, Bao;Sun, Ranran;Liu, Xuezhou;Xu, Yongjiang;Jiang, Yan;Yan, Kewen;Chen, Yan;Shi, Bao;Sun, Ranran;Liu, Xuezhou;Xu, Yongjiang;Jiang, Yan;Yan, Kewen;Chen, Yan;Shi, Bao;Liu, Xuezhou;Xu, Yongjiang;Jiang, Yan;Liu, Xuezhou
关键词：Myoblast determination proteins; Growth hormone; Insulin-like growth factor; Cloning and expression; Seriola lalandi
