文献类型： 外文期刊

作者： Bian, Chao ¹ ; Li, Rui-Han ³ ; Ruan, Zhi-Qiang ³ ; Chen, Wei-Ting ⁶ ; Huang, Yu ³ ; Liu, Li-Yue ⁷ ; Zhou, Hong-Ling ⁸ ; Chong, Cheong-Meng ⁹ ; Mu, Xi-Dong ² ; Shi, Qiong ¹ ;

作者机构： 1.Shenzhen Univ, Coll Life Sci & Oceanog, Lab Aquat Genom, Shenzhen 518057, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Key Lab Prevent & Control Aquat Invas Alien Specie, Minist Agr & Rural Affairs, Guangdong Modern Recreat Fisheries Engn Technol Ct, Guangzhou 510380, Guangdong, Peoples R China

3.BGI Acad Marine Sci, BGI Marine, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ An, Shenzhen 518081, Guangdong, Peoples R China

4.Chinese Acad Sci, Chengdu Inst Biol, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliza, Chengdu 610041, Sichuan, Peoples R China

5.Chinese Acad Sci, Chengdu Inst Biol, Ecol Restorat & Biodivers Conservat Key Lab Sichua, Chengdu 610041, Sichuan, Peoples R China

6.Jiaying Univ, Sch Life Sci, Meizhou 514015, Guangdong, Peoples R China

7.Chinese Acad Sci, China Zebrafish Resource Ctr, State Key Lab Freshwater Ecol & Biotechnol, Natl Aquat Biol Resource Ctr,Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China

8.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Agr Genom Inst Shenzhen,Shenzhen B, Shenzhen, Guangdong, Peoples R China

9.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau 999078, Macau, Peoples R China

10.Neijiang Normal Univ, Coll Life Sci, Ctr Aquat Genom, Neijiang 641100, Sichuan, Peoples R China

关键词： Glass catfish; Whole-genome sequencing; edn3b-/- -/- mutant; Transparent phenotype

期刊名称：ZOOLOGICAL RESEARCH （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 2095-8137

年卷期： 2024 年 45 卷 5 期

页码：

收录情况： SCI

摘要： Glass catfish ( Kryptopterus vitreolus) ) are notable in the aquarium trade for their highly transparent body pattern. This transparency is due to the loss of most reflective iridophores and light-absorbing melanophores in the main body, although certain black and silver pigments remain in the face and head. To date, however, the molecular mechanisms underlying this transparent phenotype remain largely unknown. To explore the genetic basis of this transparency, we constructed a chromosome-level haplotypic genome assembly for the glass catfish, encompassing 32 chromosomes and 23 344 protein- coding genes, using PacBio and Hi-C sequencing technologies and standard assembly and annotation pipelines. Analysis revealed a premature stop codon in the putative albinism-related tyrp1b gene, encoding tyrosinase-related protein 1, rendering it a nonfunctional pseudogene. Notably, a synteny comparison with over 30 other fish species identified the loss of the endothelin-3 ( edn3b ) gene in the glass catfish genome. To investigate the role of edn3b, , we generated edn3b-/- -/- mutant zebrafish, which exhibited a remarkable reduction in black pigments in body surface stripes compared to wild-type zebrafish. These findings indicate that edn3b loss contributes to the transparent phenotype of the glass catfish. Our high-quality chromosome-scale genome assembly and identification of key genes provide important molecular insights into the transparent phenotype of glass catfish. These findings not only enhance our understanding of the molecular mechanisms underlying transparency in glass catfish, but also offer a valuable genetic resource for further research on pigmentation in various animal species.