Effects of saline-alkali stress on the tissue structure, antioxidation, immunocompetence and metabolomics of Eriocheir sinensis
文献类型： 外文期刊
作者： Zhang, Rui 1 ; Zhao, Zhigang 1 ; Li, Mingshuai 1 ; Luo, Liang 1 ; Wang, Shihui 1 ; Guo, Kun 1 ; Xu, Wei 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Key Open Lab Cold Water Fish Germplasm Resources &, Harbin 150070, Peoples R China
2.CAFS, Engn Technol Res Ctr Saline Alkaline Water Fisheri, Harbin 150070, Peoples R China
3.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China
关键词： Eriocheir sinensis; Saline -alkali stress; Toxic effects; Physiological function
期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：9.8； 五年影响因子：9.6 ）
ISSN： 0048-9697
年卷期： 2023 年 871 卷
页码：
收录情况： SCI
摘要： Saline-alkali water resources are abundant and widely distributed in China. The effective utilization of saline-alkali water resources by fishery is of great significance to enhance the aquatic product economy and restore the ecology of saline-alkali environments. Eriocheir sinensis is a saline-alkali water-suitable species. To explore its physiological re-sponse to saline-alkali stress, the hepatopancreas tissue structure, antioxidation, immunocompetence and metabolo-mics were investigated after 96 h of gradient saline-alkali treatment. The results confirmed the hepatopancreas damage through tissue sectioning, abnormal enzyme activity (aspartate transaminase (AST), alanine aminotransferase (ALT)) and aberrant malondialdehyde (MDA) content. The activity of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (T-AOC) was significantly upregulated (p < 0.05), which was followed by a decrease trend, indicating the enhancement of antioxidant capacity in response to the stress. Strengthened immunocompetence in response to saline-alkali toxicity was shown in the gradual increase of immune enzyme activity (acid phosphatase (ACP) and alkaline phosphatase (AKP)) and the upregulated expression of immune genes (hsp 70, hsp 90, proPO and toll). Among the differential metabolites quantified by metabolomics, small peptides were significantly downregulated (p < 0.05), and acylcarnitines were obviously upregulated (p < 0.05), indicating that saline-alkali toxicity inhibited protein catabolism and stimulated the mobilization of energy reserves. Metabolic pathways enriched through the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that Eriocheir sinensis activated different mech-anisms in response to various degrees of stress, such as "ABC transporters" and "purine metabolism" in response to low saline-alkali stress, while "pyrimidine metabolism" and "beta-alanine metabolism" to high saline-alkali stress.
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