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Digital RNA-seq analysis of the cardiac transcriptome response to thermal stress in turbot Scophthalmus maximus

文献类型： 外文期刊

作者： Huang, Zhihui 1 ; Guo, Xiaoli 1 ; Wang, Qingmin 1 ; Ma, Aijun 1 ; Zhao, Tingting 1 ; Qiao, Xuewei 4 ; Li, Ming 4 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Shandong Key Lab Marine Fisheries Biotechnol & Ge, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China

4.Weihai Zhongfu Aquaculture Co Ltd, Weihai 264329, Peoples R China

关键词： Cardiac tissue; Digital RNA-Seq; Energy metabolism; m6A; Turbot

期刊名称：JOURNAL OF THERMAL BIOLOGY （ 2021影响因子：3.189； 五年影响因子：3.342 ）

ISSN： 0306-4565

年卷期： 2022 年 104 卷

页码：

收录情况： SCI

摘要： The hearts of fish play a major role in their physiological plasticity and acclimation to different thermal conditions. To understand the precise mechanism and the pathways activated by thermal cardiac stress in fish, we sampled cardiac tissue from juvenile turbot (Scophthalmus maximus) exposed to control (14 degrees C) and test (20 degrees C, 24 degrees C, and 28 degrees C) conditions, and performed digital RNA sequencing (RNA-seq). A total of 3359 differentially expressed genes (DEGs) were identified. The results of an expression tendency analysis and KEGG annotation analysis of the DEGs demonstrated that energy metabolism played a core role in thermal stress in turbot for the majority of the up-regulated genes. This was followed by lipid metabolism, mitochondrial function, glycolysis, and carbohydrate metabolism. RNA modifications are gaining the interest of biologists worldwide. In this study, at the transcriptome level, our results showed that 246 m6A-containing genes were detected in the DEGs, which were related to EIF3C, EIF3D, EIF3J, METTL16, RBM15B, VIRMA, and YTHDC1. This indicates that m6A is involved in the regulation of heat stress in turbot. This study is an important step towards understanding the cardiac adaptive response to thermal stress. Importantly, the plasticity of cardiac tissue could predict the adaptability of fish species to environmental temperature.

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