Quantitative Distribution and Transmission of Tea Plant Necrotic Ring Blotch Virus in Camellia sinensis
文献类型： 外文期刊
作者： Ren, Hengze 1 ; Chen, Yao 1 ; Zhao, Fumei 2 ; Ding, Changqing 1 ; Zhang, Kexin 1 ; Wang, Lu 1 ; Yang, Yajun 1 ; Hao, Xinyuan 1 ; Wang, Xinchao 1 ;
作者机构： 1.Chinese Acad Agr Sci, Tea Res Inst, Minist Agr & Rural Affairs, Key Lab Biol Genet & Breeding Special Econ Animal, Hangzhou 310008, Peoples R China
2.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Peoples R China
关键词： tea tree virus; quantitative real-time PCR; virus detection; virus transmission
期刊名称：FORESTS （ 影响因子：3.282； 五年影响因子：3.292 ）
ISSN：
年卷期： 2022 年 13 卷 8 期
页码：
收录情况： SCI
摘要： Tea plant necrotic ring blotch virus (TPNRBV), which carries four positive-sense single-stranded RNA segments, causes discoloration spots and multiple necrotic ring blotches in tea trees. To understand the distribution and transmission of TPNRBV in tea trees and prevent its spread, a SYBR Green real-time quantitative polymerase chain reaction (RT-qPCR) method for detecting the four virus segments was developed. The limit of detection of RT-qPCR was 3.81, 4.73, 3.58, and 4.64 copies/mu L for the four strands of TPNRBV, which was 100-fold more sensitive than conventional PCR for RNA1 detection, 10-fold for RNA2 and RNA3, and 1000-fold for RNA4 detection. Visual observation and RT-qPCR of different tea plant tissues showed that symptomatic mature leaves contained the highest TPNRBV load; the concentrations of the four RNAs in other tissues decreased or were undetectable with increasing distances from symptomatic leaves. TPNRBV did not spread efficiently through seeds, cuttings, or mechanical inoculation, but was transmitted to some tea cultivars, particularly light albinistic varieties such as 'Zhonghuang 1' and 'Huangjinya', under field conditions. Our TPNRBV detection method is useful for determining the distribution and transmission characteristics of TPNRBV and selecting tissues with the highest viral load for early diagnosis, monitoring, and management of the disease.
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