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Construction of G-quadruplex/thioflavin T fluorescent probe for label-free detection of malachite green

文献类型： 外文期刊

作者： Wang, Sijia 1 ; Li, Mengrong 1 ; Li, Wang 1 ; He, Kaiyu 2 ; Ren, Jiali 1 ;

作者机构： 1.Cent South Univ Forestry & Technol, Hunan Key Lab Forestry Edible Resources Safety & P, Changsha 410004, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

关键词： Malachite green; G-quadruplex; Thioflavin T; Fluorescent probe

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）

ISSN： 0026-265X

年卷期： 2024 年 207 卷

页码：

收录情况： SCI

摘要： Malachite green (MG), owing to its extreme toxicity, is strictly prohibited as a drug in aquaculture across numerous countries. To facilitate an economical and user-friendly strategy for MG detection, we rigorously screened c-MYC22 from a wide range of G-quadruplex (G4) DNAs, aiming to craft a fluorescent probe in tandem with the water-soluble fluorescent dye thioflavin T (ThT). This endeavor culminated in the development of a label-free, rapid and sensitive method for MG detection. The fluorescence of the G4/ThT complex dims significantly when MG interacts with G4, allowing for the quantitative analysis of MG. Our method boasts linear detection ranges between 50-1000 mu g/L and 400-6000 mu g/L, with an impressive limit of detection as low as 6.82 mu g/L and a short detection time of 15 min. The fluorescent probe demonstrates excellent specificity, rendering it ideal for MG detection in real water samples. Furthermore, this method stands out for its simplicity and efficiency, the probe used does not need to be modified, and the detection takes only one step with a short detection time. These features make it a strong contender for commercialization as a method of MG detection, presenting significant potential for further advancement.

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