Hue-change coupled with CRISPR-Cas12a lateral flow assay for the semiquantitative detection of Salmo salar adulteration
文献类型： 外文期刊
作者： Yuan, Jingrui 1 ; Wang, Liu 1 ; He, Fang 1 ; Huang, Liang 2 ; He, Kaiyu 1 ; Wang, Hongmei 1 ; Wang, Jing 2 ; Xu, Xiahong 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Informat Traceabil Agr Prod,Minist Agr & R, Hangzhou 310021, Peoples R China
2.Zhejiang Univ Technol, Coll Chem Engn, Hangzhou 310014, Peoples R China
3.UCAS, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310024, Peoples R China
关键词： Hue change; CRISPR-Cas12a; Lateral flow assay; Quantitative analysis; Salmo salar adulteration
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 463 卷
页码：
收录情况： SCI
摘要： Salmo salar is one of the most popular salmon species due to its meaty texture and quality protein. Oncorhynchus mykiss, which has a muscle texture similar to that of Salmo salar and is less expensive, is often used as a substitute for Salmo salar. As Salmo salar and Oncorhynchus mykiss belong to the same subfamily of Salmonidae, traditional methods are ineffective in the specific detection of the two. In this study, we combined hue-change with CRISPR/Cas12a lateral flow assay to detect the Salmo salar adulteration. This method detected S. salar genomic DNA at a vLOD of 5 copies, and was able to accurately identify adulterated samples containing 5 % w/w Salmo salar within one hour. In addition, the detection of Salmo salar in processed food products was achieved with the naked-eye at a concentration range of 0 % similar to 70 % w/w, and the detection accuracy is between 93.3 % similar to 100 %.
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