文献类型： 外文期刊

作者： Yu, Siriguleng ¹ ; Liu, Chaoqun ¹ ; Lv, Hong ¹ ; Guo, Zhijun ¹ ; Yao, Hongqiang ¹ ; Wen, Xin ¹ ; Xiu, Ying ³ ; Gao, Wa ⁴ ; Li, Ziyi ¹ ; Yu, Wen ¹ ; Niu, Yaru ¹ ; Jin, Junjian ¹ ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Vet Med, Hohhot, Peoples R China

2.Minist Agr, Key Lab Clin Diag & Treatment Technol Anim Dis, Hohhot, Peoples R China

3.Inner Mongolia Ctr Dis Control & Prevent, Dept Publ Hlth, Hohhot, Peoples R China

4.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China

关键词： Bactrian camel; exosome; metabolism; miR-144-5p; PGC-1 alpha

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN：

年卷期： 2025 年 12 卷

页码：

收录情况： SCI

摘要： Introduction MiR-144-5p is differentially expressed in plasma exosomes from Bactrian camels of varying body sizes, with GO and KEGG analyses indicating that its target genes play crucial roles in lipid metabolism. PGC-1 alpha, confirmed as a key target through RNA pull-down and dual-luciferase reporter assays, is a significant regulator of this process. This study aims to investigate the impact of miR-144-5p on lipid metabolism in a mouse model of high-fat diet (HFD)-induced obesity to elucidate the mechanistic pathways involved.Methods Male C57BL/6 mice were assigned to groups and fed either a control diet or an HFD for 12 weeks, which was followed by a 3-week intervention with miR-144-5p. Various metabolic parameters and gene expressions were evaluated to determine the effects of this treatment.Results The treatment significantly reduced diet-induced adiposity and decreased inflammatory responses. Additionally, it inhibited PGC-1 alpha expression in the liver tissue of HFD-fed mice. There was an increase in CPT1 activity and fatty acid beta-oxidation rate, along with the downregulation of FASN mRNA expression and the upregulation of CPT1 and ACOX1 expression, promoting fatty acid oxidation.Discussion These findings suggest that miR-144-5p exerts anti-obesity effects by enhancing fatty acid oxidation, likely through modulation of the PGC-1 alpha/AMPK signaling pathway. This study provides new insights into potential miRNA-based therapeutic strategies for obesity and related metabolic disorders.