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Colorimetric and Reverse Fluorescence Dual-Signal Readout Immunochromatographic Assay for the Sensitive Determination of Sibutramine

文献类型： 外文期刊

作者： Gui, Yun 1 ; Zhao, Yun 2 ; Liu, Pengyan 2 ; Wang, Yulong 2 ; Mao, Xinxin 2 ; Peng, Chifang 3 ; Hammock, Bruce D. 5 ; Zhang, Cunzheng 1 ;

作者机构： 1.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol,Inst Food Safety & Nutr, Nanjing 210014, Peoples R China

3.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

4.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

5.Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA

6.Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA

期刊名称：ACS OMEGA （ 影响因子：4.1； 五年影响因子：4.0 ）

ISSN： 2470-1343

年卷期： 2024 年 9 卷 6 期

页码：

收录情况： SCI

摘要： Later flow immunochromatographic assay has been widely used in clinical, environmental, and other diagnostic applications owing to its high sensitivity and throughput. However, most immunoassays operate in the "turn-off" mode for detecting targets of low molecular weight. The signal intensity decreases as the analyte concentration increases, which poses a challenge for achieving ultrasensitive detection at low concentrations and is counterintuitive to new users. In this work, a fluorometric immunochromatographic assay (FICA) is developed to simultaneously read "turn-on" fluorescent and "turn-off" colorimetric signals, where ZnCdSe/ZnS quantum dots act as fluorescence donors and gold nanoparticles (AuNPs) act as quenchers. The fluorescent signal (excitation/emission wavelengths of 365/525 nm) is positively correlated with analytes' concentration. Taking sibutramine (SBT) as the analysis target, the visual limit of detection for SBT reached 3.9 ng/mL, and the limit of Quantitation was 5.0 ng/mg in spiked samples. The developed FICA achieves a high sensitivity in SBT detection, which is much lower than that of the colloidal gold-based immunochromatographic assay. This dual-function detection mode has great potential to be used as a rapid on-site semiquantitative method, providing an alternative mode for the determination of low levels of target analytes.

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