文献类型： 外文期刊

作者： Xu, Sheng-Jun ¹ ; Zhang, Jin-Kui ¹ ; Hou, Rui-Ning ⁴ ; Jing, Zhuo-Qiong ¹ ; Sun, Qian ¹ ; Zhu, Xiao ⁵ ; Fu, Li-Xin ⁶ ; Zhang, Feng-Yi ⁷ ; Ren, Hong-Lei ⁷ ; Guo, Zhi-Jie ¹ ;

作者机构： 1.Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou 730070, Peoples R China

2.Minist Agr, Sci Observing & Expt Stn Crop Pests Tianshui, Gangu 741299, Peoples R China

3.Gansu Agr Univ, Coll Plant Protect, Lanzhou 730070, Peoples R China

4.Heilongjiang Acad Agr Sci, Hort Sub Acad, Harbin 150069, Peoples R China

5.Heilongjiang Univ, Harbin 150086, Peoples R China

6.Heilongjiang Acad Agr Sci, Maize Res Inst, Harbin 150086, Peoples R China

7.Heilongjiang Acad Agr Sci, Soybean Res Inst, Harbin 150086, Peoples R China

关键词： Bacillus atrophaeus; Antagonistic activity; Root rot; Fusarium solani; Phytophthora capsici; Capsicum annuum

期刊名称：TROPICAL PLANT PATHOLOGY （ 影响因子：2.5； 五年影响因子：2.2 ）

ISSN： 1983-2052

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The aim of the present study was to screen native cultivable rhizobacteria with potential applications for biocontrol of root rot, which results in a serious and persistent disease problem for the pepper industry worldwide and is difficult to control through chemical and agricultural means. A total of 47 bacteria were isolated from pepper rhizosphere soil by spread plate and screened by dual culture assay. In in vitro dual culture assay, the strain DX1708 showed strong antagonistic activity against Fusarium oxysporum, F. solani, F. verticillioides, Phytophthora capsici and Rhizoctonia solani. Based on cultural and morphological characterization, and 16S rRNA gene and gyrA gene phylogenetic analysis strain DX1708 was identified as Bacillus atrophaeus. Strain DX1708 demonstrated many characteristics that are beneficial for plants, such as production of alpha-amylase, ammonia, cellulase, indole-3-acetic acid, pectinase, protease, siderophores, and phosphate solubilization. PCR-based detection of antimicrobial lipopeptides and peptides gene revealed the presence of ten genes in B. atrophaeus strain DX1708, which code for enzymes involved in production of bacilysin, fengycin, mycosubtilin, subtilosin and surfactin. In planta application of B. atrophaeus strain DX1708 significantly decreased root rot severity compared to untreated controls under greenhouse conditions. The strain DX1708 was tested in the field during three successive years (2018-2020). The results demonstrated a significant decrease in disease severity. These results suggest that B. atrophaeus strain DX1708 could control plant diseases via direct and indirect modes of action and can be used as a potential agent for biological control.