Integrated miRNA-mRNA analysis uncovers immediate-early response to salinity stress in gill-derived cell line of Gymnocypris przewalskii
文献类型： 外文期刊
作者： Wei, Fulei 1 ; Zuo, Xianzhi 1 ; Jin, Faxin 1 ; Yang, Qiangdong 1 ; Cui, Yanrong 2 ; Zhao, Mingyang 1 ; Cui, Mingming 3 ; Liang, Jian 2 ;
作者机构： 1.Qinghai Univ, Coll Ecoenvironm Engn, 251 Ningda Rd, Xining 810016, Peoples R China
2.Qinghai Univ, Coll Ecoenvironm Engn, State Key Lab Plateau Ecol & Agr, 251 Ningda Rd, Xining 810016, Peoples R China
3.Qinghai Univ, Qinghai Acad Agr & Forestry Sci, Xining 810016, Peoples R China
关键词： Gymnocypris przewalskii; Salinity; Cell line; Apoptosis; miRNA; Transcriptome
期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）
ISSN： 1471-2164
年卷期： 2024 年 25 卷 1 期
页码：
收录情况： SCI
摘要： Salinity adaptation is an important issue in aquaculture. Understanding the immediate-early response to salinity stress helps in comprehending this process. In vitro experiments using cell lines can explain cell-independent reactions without the involvement of hormones in vivo. In this study, salinity stress experiments were conducted using cell line derived from the gills of Gymnocypris przewalskii (GPG cell line) to isolate immediate-early response-related genes and miRNAs using transcriptomics, followed by bioinformatics analysis. The results showed that intracellular free Ca2+ appeared to be a key factor in cell sensing and initiating downstream cell signaling in response to external salinity. Additionally, cell apoptosis was the most common feature of salinity stress, with multiple signaling pathways involved in salinity-induced cell apoptosis. Furthermore, MiRNAs played a crucial role in the rapid response to salinity stress by selectively inhibiting the expression of specific genes. Additionally, for the first time in the G. przewalskii genome, Tf2 and TY3 families of transposons were found to have responsive roles to the external salinity stress. This study contributes to a better understanding of osmotic sensing in G. przewalskii and provides theoretical assistance for improving salinity adaptation in aquaculture fish species.
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