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An ABCG-type transporter intensifies ametryn catabolism by Phase III reaction mechanism in rice

文献类型： 外文期刊

作者： Qiao, Yuxin 1 ; Zhang, A. I. Ping 1 ; Ma, Li Ya 1 ; Zhang, Nan 1 ; Liu, Jintong 1 ; Yang, Hong 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Sci, Jiangsu Key Lab Pesticide Sci, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, State & Local Joint Engn Res Ctr Green Pesticide I, Nanjing 210095, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjing 210014, Peoples R China

4.Nanjing Agr Univ, Coll Sci, Weigang 1, Chem Bldg, Nanjing 210095, Peoples R China

关键词： ABC transporter; Ametryn; Metabolism; Rice

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2023 年 457 卷

页码：

收录情况： SCI

摘要： Pesticide residues in food crops are one of the seriously environmental contaminants that risk food safety and human health. Understanding the mechanism for pesticide catabolism is critical to develop effective bio-techniques for rapid eliminating the residues in food crops. In this study we characterized a novel ABC trans-porter family gene ABCG52 (PDR18) in regulating rice response to pesticide ametryn (AME) widely used in the farmland. Efficient biodegradation of AME was evaluated by measuring its biotoxicity, accumulation, and me-tabolites in rice plants. OsPDR18 was localized to the plasma membrane and strongly induced under AME exposure. Transgenic rice overexpressing OsPDR18 (OE) conferred rice resistance and detoxification to AME by increasing chlorophyll contents, improving growth phenotypes, and reducing AME accumulation in plants. The AME concentrations in OE plants were only 71.8-78.1% (shoots) and 75.0-83.3% (roots) of the wild type. Mutation of OsPDR18 by CRISPR/Cas9 protocol led to the compromised growth and enhanced AME accumu-lation in rice. Five AME metabolites for Phase I and 13 conjugates for Phase II reactions in rice were charac-terized by HPLC/Q-TOF-HRMS/MS. Relative content analysis revealed that the AME metabolic products in OE plants were significantly reduced compared with wild-type. Importantly, the OE plants accumulated less AME metabolites and conjugates in rice grains, suggesting that OsPDR18 expression may actively facilitate the transport of AME for catabolism. These data unveil a AME catabolic mechanism by which OsPDR18 contributes to the AME detoxification and degradation in rice crops.

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