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Isolation, identification and genetic characterization analysis of a fowl aviadenovirus serotype 4 strain from Tianjin, China

文献类型： 外文期刊

作者： Wang, Lili 1 ; Zheng, Li 1 ; Jiang, Shan 1 ; Li, Xiuli 1 ; Lu, Chao 1 ; Zhang, Li 1 ; Ren, Weike 1 ; Li, Cheng 1 ; Tian, Xia 1 ;

作者机构： 1.Tianjin Acad Agr Sci, Anim Husb & Vet Res Inst, Tianjin 300381, Peoples R China

2.Minist Agr & Rural Affairs China, Tianjin Sci Observat Expt Stn Vet Med & Diag Tech, Tianjin 300381, Peoples R China

3.Tianjin Engn Res Ctr Livestock & Poultry Hlth Bre, Tianjin, Peoples R China

关键词： Fowl aviadenovirus serotype 4 (FAdV-4); Isolation and identification; Pathogenicity; Phylogenetic analysis

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

ISSN： 1567-1348

年卷期： 2021 年 96 卷

页码：

收录情况： SCI

摘要： A fowl aviadenovirus serotype 4 (FAdV-4), Y17215-1, was isolated from the liver of chickens with Hydropericardium-hepatitissyndrome(HHS) in a chicken farm of Tianjin, China. Obvious cytopathic effects were observed in the infected chicken liver hepatocellular carcinoma cell line (LMH cells) at 24 h post infection (hpi), which consisted of enlarger and rounder shape of cells. The typical and specific green fluorescence was observed by indirect immunofluorescence assay (IFA). Tissue Culture Infectious Dose50 (TCID50) of it measured after five stable passage in LMH cells reached 10(6.5)TCID(50)/0.1 mL. The strain was inoculated through allantoic membrane of 10-day specific pathogen free(SPF) Chick embryos, the thicker allantoic membranes were observed at 120 hpi. 7-day-old SPF chickens were inoculated with the strain via intramuscular (i.m.) or intranasal (i.n.) injection which resulted in 100% mortality of test chickens. Additionally, the sickness and death of cohabitation chickens in the test group were observed which indicated that the virus can infect healthy chickens by horizontal transmission. The sick chickens showed depression, anorexia and diarrhea with green watery feces. Y17215-1-inoculated chickens mainly presented swollen liver with blood spot, and the enhancement of effusion or yellow gel like effusion that were observed in the pericardium through necropsy. Histopathological examination showed focal necrosis of hepatocytes and characteristic eosinophilic inclusion bodies in the cytoplasm. The results showed that the Y17215-1 isolate had high pathogenicity to SPF chickens. The phylogenetic analysis of the major structural proteins including hexon, fiber-1 and fiber-2 revealed that Y17215-1 strain belongs to C species of fowl aviadenovirus of aviadenovirus family, and has high homology with other Chinese strains isolated in recent years, but was distinct from ON1, MX-SHP95, KR5 and other foreign isolates. This study laid a foundation for further study of epidemiological investigation, pathogenic mechanism as well as the diagnosis and control technology of FAdV-4.

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