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Development and characterization of a new cell line from brain tissues of the rare minnow (Gobiocypris rarus)

文献类型： 外文期刊

作者： Lv, Yuefeng 1 ; Wang, Yingying 1 ; Wang, Qing 1 ; Li, Yingying 1 ; Yin, Jiyuan 1 ; Yang, Guang 2 ; Shi, Cunbin 1 ; Li, Bo 1 ; Wang, Yahui 1 ; Bergmann, Sven M. 3 ; Zeng, Weiwei 4 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol Guangdong Prov, Key Lab Fishery Drug Dev,Minist Agr, Guangzhou, Peoples R China

2.Tianjin Agr Univ, Coll Fisheries, Tianjin, Peoples R China

3.Friedrich Loeffler Inst FLI, Inst Infectol, Fed Res Inst Anim Hlth, Greifswald, Germany

4.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise, Key Lab Anim Mol Design & Precise Breeding Guangd, Foshan, Peoples R China

关键词： cell line; Gobiocypris rarus; GrB cells; tilapia lake virus (TiLV)

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.184； 五年影响因子：2.447 ）

ISSN： 1355-557X

年卷期： 2022 年 53 卷 5 期

页码：

收录情况： SCI

摘要： A permanent epithelial-like cell line, named GrB, was established from brain tissues of the rare minnow (Gobiocypris rarus). GrB cells grow well at temperatures between 22celcius and 27celcius in L-15 medium supplemented with 10% FBS. Chromosome analysis revealed that GrB cells possessed a diploid chromosome number of 2n = 50 at passage 50, consistent with the number of chromosomes reported for G. rarus. Partial amplification and sequence analysis of the cytochrome oxidase subunit I (COI) confirmed that GrB cells are originated from G. rarus. The cell lines also successfully expressed green fluorescence protein from plasmid pEGFP-N1 and which makes them suitable for transfection studies. A cytolytic cytopathic effect was observed in the cells after infection with tilapia lake virus 2017A (TiLV-2017A). A large number of virus particles were detected by electron microscopy. Immunofluorescence and virus titration assays indicated that TiLV-2017A titres reached 10(5.67) TCID50/ml at 7 days post-infection (dpi). Healthy adult rare minnows were challenged with virus obtained from these cells showed that they can be infected by TiLV-2017A, which causes low mortality but clinical signs. The GrB cell line may represent a valuable model as basics for fish virology and fish immunology.

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