Molecular cloning and functional characterization of sarco/endoplasmic reticulum Ca2+-ATPase from Chinese mitten crab (Eriocheir sinensis)
文献类型： 外文期刊
作者： Yu, Junjie 1 ; Feng, Wenrong 2 ; Chen, Xue 2 ; Song, Changyou 2 ; Su, Shengyan 1 ; Ge, Jiachun 3 ; Tang, Yongkai 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr & Rural Affairs, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi, Jiangsu, Peoples R China
3.Freshwater Fisheries Res Inst Jiangsu Prov, Nanjing, Peoples R China
关键词： calcium signalling; Eriocheir sinensis; moulting; sarco; endoplasmic reticulum Ca2+-ATPases
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.184； 五年影响因子：2.447 ）
ISSN： 1355-557X
年卷期： 2022 年 53 卷 13 期
页码：
收录情况： SCI
摘要： Ca2+ plays an important role in signal transduction and regulates various physiological functions of cells. The active transport of Ca2+ from the cytosol to the lumen of the sarcoplasmic or endoplasmic reticulum occurs via sarco/endoplasmic reticulum Ca2+-ATPases (SERCA). However, the function of this protein during moulting cycles remains unclear in Chinese mitten crab (Eriocheir sinensis). In this study, we cloned a cDNA encoding a putative SERCA protein from the Y-organs (moulting glands) in E. sinensis. The cloned Es-SERCA cDNA (4552 bp) included an open reading frame of 3003 bp encoding a 1000-residue protein with a predicted molecular mass of 109.60 kDa. The predicted Es-SERCA protein was clustered with those from other arthropods in phylogenetic analysis. Further, qPCR revealed that Es-SERCA was widely expressed in in all tested tissues with the highest expression in the muscle, followed by the eyestalks, intestines, hepatopancreas, Y-organs and gills. The stage-specific fluctuations among different tissues indicated a functional role of SERCA in calcium homeostasis. The relative mRNA level of the Es-SERCA in Y-organs during the moulting cycle was similar to the level trend of ecdysteroid concentration in haemolymph, indicating that Ca2+ signalling stimulated ecdysteroid synthesis in Y-organs. Altogether, our study provides a reference for further research on the molecular mechanisms of the moulting in E. sinensis.
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