文献类型： 外文期刊

作者： Xu, Yingwei ¹ ; Ni, Ling ¹ ; Guan, Huiqiong ¹ ; Chen, Dailing ¹ ; Qin, Si ³ ; Chen, Lanming ¹ ;

作者机构： 1.Shanghai Ocean Univ, Coll Food Sci & Technol, Key Lab Qual & Safety Risk Assessment Aquat Prod S, Minist Agr & Rural Affairs Peoples Republ China, Shanghai 201306, Peoples R China

2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

3.Hunan Agr Univ, Coll Food Sci & Technol, Lab Food Funct & Nutrigen, Changsha 410128, Peoples R China

关键词： Klebsiella pneumoniae; genetic diversity; virulence; antibiotic resistance; heavy metal tolerance; aquatic animal; food safety

期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）

ISSN：

年卷期： 2022 年 11 卷 24 期

页码：

收录情况： SCI

摘要： Klebsiella pneumoniae can cause serious pneumonitis in humans. The bacterium is also the common causative agent of hospital-acquired multidrug-resistant (MDR) infections. Here we for the first time reported the genetic diversity of K. pneumoniae strains in 14 species of edible aquatic animals sampled in the summer of 2018 and 2019 in Shanghai, China. Virulence-related genes were present in the K. pneumoniae strains (n = 94), including the entB (98.9%), mrkD (85.1%), fimH (50.0%), and ybtA (14.9%) strains. Resistance to sulfamethoxazole-trimethoprim was the most prevalent (52.1%), followed by chloramphenicol (31.9%), and tetracycline (27.7%), among the strains, wherein 34.0% had MDR phenotypes. Meanwhile, most strains were tolerant to heavy metals Cu2+ (96.8%), Cr3+ (96.8%), Zn2+ (91.5%), Pb2+ (89.4%), and Hg2+ (81.9%). Remarkably, a higher abundance of the bacterium was found in bottom-dwelling aquatic animals, among which mollusk Tegillarca granosa contained K. pneumoniae 8-2-5-4 isolate from serotype K2 (ST-2026). Genome features of the potentially pathogenic isolate were characterized. The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR)-based genome fingerprinting classified the 94 K. pneumoniae strains into 76 ERIC genotypes with 63 singletons, demonstrating considerable genetic diversity in the strains. The findings of this study fill the gap in the risk assessment of K. pneumoniae in edible aquatic animals.