MoOpy2 is essential for fungal development, pathogenicity, and autophagy in Magnaporthe oryzae
文献类型： 外文期刊
作者： Cai, Ying-Ying 1 ; Wang, Jing-Yi 1 ; Wu, Xi-Yu 1 ; Liang, Shuang 2 ; Zhu, Xue-Ming 3 ; Li, Lin 3 ; Lu, Jian-Ping 4 ; Liu, Xiao-Hong 1 ; Lin, Fu-Cheng 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Managing Biot & Chem Treats Qual &, Hangzhou 310058, Peoples R China
2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Cent Lab, Hangzhou 310021, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Treats Qual &, Hangzhou 310021, Peoples R China
4.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Peoples R China
期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.476； 五年影响因子：6.82 ）
ISSN： 1462-2912
年卷期： 2022 年 24 卷 3 期
页码：
收录情况： SCI
摘要： The development and pathogenicity of the fungus Magnaporthe oryzae, the causal agent of destructive rice blast disease, require it to perceive external environmental signals. Opy2, an overproduction-induced pheromone-resistant protein 2, is a crucial protein for sensing external signals in Saccharomyces cerevisiae. However, the biological functions of the homologue of Opy2 in M. oryzae are unclear. In this study, we identified that MoOPY2 is involved in fungal development, pathogenicity, and autophagy in M. oryzae. Deletion of MoOPY2 resulted in pleiotropic defects in hyphal growth, conidiation, germ tube extension, appressorium formation, appressorium turgor generation, and invasive growth, therefore leading to attenuated pathogenicity. Furthermore, MoOpy2 participates in the Osm1 MAPK pathway and the Mps1 MAPK pathway by interacting with the adaptor protein Mst50. The interaction sites of Mst50 and MoOpy2 colocalized with the autophagic marker protein MoAtg8 in the preautophagosomal structure sites (PAS). Notably, the Delta Moopy2 mutant caused cumulative MoAtg8 lipidation and rapid GFP-MoAtg8 degradation in response to nitrogen starvation, showing that MoOpy2 is involved in the negative regulation of autophagy activity. Taken together, our study revealed that MoOpy2 of M. oryzae plays an essential role in the orchestration of fungal development, appressorium penetration, autophagy and pathogenesis.
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