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Antibody Generation and Rapid Immunochromatography Using Time-Resolved Fluorescence Microspheres for Propiconazole: Fungicide Abused as Growth Regulator in Vegetable

文献类型： 外文期刊

作者： Chen, Bo 1 ; Shen, Xing 1 ; Li, Zhaodong 2 ; Wang, Jin 1 ; Li, Xiangmei 1 ; Xu, Zhenlin 1 ; Shen, Yudong 1 ; Lei, Yi 3 ; Huang, Xinan 4 ; Wang, Xu 6 ; Lei, Hongtao 1 ;

作者机构： 1.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

2.South China Agr Univ, Coll Mat & Energy, Guangzhou 510642, Peoples R China

3.Guangdong Inst Food Inspect, Zengcha Rd, Guangzhou 510435, Peoples R China

4.Guangzhou Univ Chinese Med, Trop Med Inst, Guangzhou 510405, Peoples R China

5.Guangzhou Univ Chinese Med, South China Chinese Med Collaborat Innovat Ctr, Guangzhou 510405, Peoples R China

6.Guangdong Acad Agr Sci, Inst Qual Stand & Monitoring Technol Agroprod, Guangzhou 510405, Peoples R China

关键词： propiconazole; hapten; antibody; time-resolved fluorescence; lateral flow immunochromatographic assay

期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）

ISSN：

年卷期： 2022 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Propiconazole (PCZ) is a fungicide popularly used to prevent and control wheat and rice bakanae disease, etc. However, it was recently found to be illegally employed as a plant regulator to induce thick stems and dark green leaves of Brassica campestris, a famous vegetable in Guangdong, South China. Due to a lack of available recognition molecules to the target analyte, it is still a big challenge to establish a rapid surveillance screening method. In this study, a novel chiral hapten was rationally designed, and an artificial immunogen was then prepared for the generation of a specific antibody against propiconazole for the first time. Using the obtained antibody, a highly sensitive time-resolved fluorescence microspheres lateral flow immunochromatographic assay (TRFMs-LFIA) was established with a visual limit of detection of 100 ng/mL and a quantitative limit of detection of 1.92 ng/mL for propiconazole. TRFMs-LFIA also exhibited good recoveries ranging from 78.6% to 110.7% with coefficients of variation below 16%. The analysis of blind real-life samples showed a good agreement with results obtained using HPLC-MS/MS. Therefore, the proposed method could be used as an ideal screening surveillance tool for the detection of propiconazole in vegetables.

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