Occurrence and distribution of Cucumber mosaic virus infecting Danshen (Salvia miltiorrhiza) in China
文献类型： 外文期刊
作者： Wu, Z. M. 1 ; Chen, Y. 2 ; Xie, X. L. 3 ; Wen, C. X. 3 ; Chen, J. F. 1 ; Zhou, M. G. 2 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
2.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
3.Hebei Acad Agr & Forestry Sci, Inst Econ Crop Res, Shijiazhuang 050051, Peoples R China
期刊名称：PHYTOPARASITICA （ 影响因子：1.439； 五年影响因子：1.569 ）
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收录情况： SCI
摘要： Cucumber mosaic virus (CMV) has resulted in much damage to Danshen (Salvia miltiorrhiza Bunge) crops in China. A 5-year survey was conducted in Hebei, Shandong, Sichuan, Shanxi, Henan and Gansu Provinces, all major Danshen-growing areas. A total of 156 Danshen plant samples with CMV symptoms were collected and tested for the presence of CMV by a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using polyclonal antibodies against CMV and a positive control, according to the supplier's instructions (Agdia). They were confirmed to be CMV by amplification of complete coat protein gene and analysis of the gene sequence. The results showed that 122 samples were infected by CMV and all of these CMV isolates belonged to subgroup IB.
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