您好,欢迎访问河北省农林科学院 机构知识库!

Generation of transgenic wheat lines with altered expression levels of 1Dx5 high-molecular weight glutenin subunit by RNA interference

文献类型: 外文期刊

作者: Yue, S. J. 1 ; Li, H. 2 ; Li, Y. W. 1 ; Zhu, Y. F. 1 ; Guo, J. K. 1 ; Liu, Y. J. 1 ; Chen, Y. 1 ; Jia, X. 1 ;

作者机构: 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Food & Oil Crops, Shijiazhuang 050031, Hebei Province, Peoples R China

3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

关键词: wheat;RNAi;HMW-GS;gene silencing

期刊名称:JOURNAL OF CEREAL SCIENCE ( 影响因子:3.616; 五年影响因子:3.891 )

ISSN: 0733-5210

年卷期: 2008 年 47 卷 2 期

页码:

收录情况: SCI

摘要: In recent years, high molecular weight glutenin subunit (HMW-GS) null mutants have been found to be useful for studying the contribution of HMW-GS to the flour processing quality of wheat (Triticum aestivum L. em. Thell.). However, few reports have dealt with the development and characterization of such variants. In the present study, the RNA interference (RNAi) method was applied to Bobwhite wheat, which has five actively expressed HMW-GS genes (namely1Ax2*,1Dx5,1Bx7,1By9, 1Dy10), with the aim of silencing the expression of 1Dx5. Out of the six transgenic events characterized,1Dx5 expression was completely blocked in four transgenic events (L I -L4), and partially reduced in the other two (L5, L6). In contrast, the protein levels of 1Ax2*, 1By9 and 1Dy 10 were not significantly affected in any of the six transgenic events. Interestingly, 1Bx7 protein accumulation was negatively affected in all six events and their progenies.1Dx5 transcript levels in developing seeds at 15 days after pollination (DAP) were undetectable in L1 and dramatically reduced in L5. The silencing of 1Dx5 expression caused a substantial decrease in flour processing quality based on Farinograph, gluten and Zeleny tests. Collectively, our data suggest that RNAi is useful for silencing HMW-GS genes. The resultant transgenic lines are of value for studying the contributions of specific HMW-GS to wheat flour processing quality. (c) 2007 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]小麦×玉米杂交双单倍体后代的HMW-GS组成和易位分析. 庞建周,王雪征,茜晓哲,王晨阳,陈淑萍. 2016

[2]Molecular cytogenetic analysis of intergeneric chromosomal translocations between wheat (Triticum aestivum L.) and Dasypyrum villosum arising from tissue culture. Li, HJ,Guo, BH,Li, YW,Du, LQ,Jia, X,Chu, CC. 2000

[3]Effects of the wheat UDP-glucosyltransferase gene TaUGT-B2 on Agrobacterium-mediated plant transformation. Zhou, Xiaohong,Wang, Ke,Du, Lipu,Lin, Zhishan,Ye, Xingguo,Liu, Yongwei.

[4]Homoeologous cloning of omega-secalin gene family in a wheat 1BL/1RS translocation. Chai, JF,Liu, X,Jia, JZ. 2005

[5]EFFECTS OF SOIL DROUGHT STRESS ON PLANT REGENERATION EFFICIENCY AND ENDOGENOUS HORMONE LEVELS OF IMMATURE EMBRYOS IN WHEAT (&ITTRITICUM AESTIVUM&IT L.). Bie, Xiaomin,Wang, Ke,Liu, Chang,Du, Lipu,Mao, Xinguo,Ye, Xingguo,Bie, Xiaomin,Liu, Yongwei. 2017

[6]Fate of labeled urea-N-15 as basal and topdressing applications in an irrigated wheat-maize rotation system in North China Plain: I winter wheat. Jia, Shulong,Wang, Xiaobin,Dai, Kuai,Zhao, Quansheng,Zhang, Xiaoming,Zhang, Dingchen,Feng, Zonghui,Wu, Xueping,Cai, Dianxiong,Jia, Shulong,Wang, Xiaobin,Dai, Kuai,Zhao, Quansheng,Zhang, Xiaoming,Zhang, Dingchen,Feng, Zonghui,Wu, Xueping,Cai, Dianxiong,Jia, Shulong,Yang, Yunma,Meng, Chunxiang,Sun, Yanming,Grant, Cynthia.

[7]Diversity of Growth Habits and Their Association with VRN Allele of 81 American Wheat Lines. Ji Gui-su,Zhang Qing-jiang,Bai Gui-hua. 2010

[8]Identification and characterization of a high kernel weight mutant induced by gamma radiation in wheat (Triticum aestivum L.). Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Zhao, Aiju. 2015

[9]Cloning and characterization of the Na+/H+ antiport genes from Triticum aestivum. Wang, ZN,Zhang, JS,Guo, BH,He, SJ,Tian, AG,Chen, SY. 2002

[10]Transformation of wheat with a gene encoding for the betaine aldehyde dehydrogenase (BADH). Guo, BH,Zhang, YM,Li, HJ,Du, LQ,Li, YX,Zhang, JS,Chen, SY,Zhu, ZQ. 2000

[11]Involvement of calcium-calmodulin in the expression of hsp26 gene in wheat. Liu, HT,Zhao, H,Li, B,Sun, DY,Zhou, RG. 2001

[12]A procedure allowing up to eight generations of wheat and nine generations of barley per annum. Zheng, Z.,Chen, G. D.,Liu, C. J.,Zheng, Z.,Yan, G. J.,Zheng, Z.,Yan, G. J.,Zheng, Z.,Wang, H. B.,Chen, G. D..

[13]Enhancing Fusarium crown rot resistance by pyramiding large-effect QTL in common wheat (Triticum aestivum L.). Zheng, Zhi,Gao, Shang,Liu, Chunji,Zheng, Zhi,Zheng, Zhi,Yan, Guijun,Liu, Chunji,Gao, Shang,Zhou, Meixue,Gao, Shang,Zhou, Meixue.

[14]Wheat acclimate to water deficit by modifying carbohydrates metabolism, water use efficiency, and growth. Hu, MengYun,Li, Hui,Shi, ZhiGang,Xu, Ping,Zhang, Zhengbin.

[15]Effects of calmodulin on DNA-binding activity of heat shock transcription factor in vitro. Li, B,Liu, HT,Mu, RL,Sun, DY,Zhou, RG.

[16]Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background. Chai, JF,Wu, ZM,Zhao, H,Laroche, A,Wang, HB.

作者其他论文 更多>>