Induction of somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants of Eruca sativa Mill.
文献类型： 外文期刊
作者： Zhang, T 1 ; Cao, ZY 2 ; Wang, XY 3 ;
作者机构： 1.Lanzhou Univ, Inst Cell Biol, Lanzhou 730000, Peoples R China
2.Gansu Acad Agr Sci, Inst Ind Crops, Lanzhou 730070, Peoples R China
3.Gansu Acad Agr Sci, Sch Life Sci & Technol, Lanzhou 730070, Peoples R China
关键词： embryogenic callus induction;plant growth regulators;plantlets;somatic embryos;DESICCATION;CULTURES;EMBRYOS;GROWTH
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）
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收录情况： SCI
摘要： A protocol was developed for high frequency somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants of Eruca sativa. Explants grown on Murashige and Skoog (MS) medium supplemented with 4.52 mu M 2,4-D formed embryogenic callus after 4 wk of culture. Secondary somatic embryos were also produced from primary somatic embryos on MS medium containing 0.56 mu M 2,4-D. Somatic embryos developed into mature embryos on MS medium in the presence of 45 g l(-1) polyethylene glycol. After desiccation, somatic embryos developed into plantlets by culturing the mature somatic embryos on 1/2xMS medium containing 0.24 mu M indole-3-butyric acid.
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