Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects
文献类型： 外文期刊
作者： Zhang, T 1 ; Wang, XY 2 ; Cao, ZY 3 ;
作者机构： 1.Lanzhou Univ, Inst Cell Biol, Lanzhou 730000, Peoples R China
2.Gansu Acad Agr Sci, Lanzhou 730070, Peoples R China
3.Gansu Agr Univ, Sch Life Sci & Technol, Lanzhou 730070, Peoples R China
关键词： growth regulators;direct organogenesis;morphological abnormalities
期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）
ISSN： 0006-3134
年卷期： 2005 年 49 卷 3 期
页码：
收录情况： SCI
摘要： A rapid plantlet regeneration system for Perilla frutescens was established from cotyledon and hypocotyl explants. A maximum of 91.06 % cotyledon and 76.4 % hypocotyl explants could directly produce shoots (3.09 +/- 0.18 shoots per explants) on Murashige and Skoog (MS) medium. The optimum hormone combinations were 4.44 mu M 6-benzylaminopurine (BA) for cotyledon and 2.22 mu M BA + 2.85 mu M indole-3-acetic acid (IAA) for hypocotyls. Rooting was induced on half-strength hormone-free MS medium. After transplantation to soil, approximate 80 % of the regenerated plantlets could survive, flower and fruit. Moreover, some morphological abnormalities were found among the regenerated plants.
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