文献类型： 外文期刊

作者： Wu, YL ¹ ; Yi, GJ ¹ ; Yang, H ¹ ; Zhou, BR ¹ ; Zeng, JW ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Pomol Res Inst, Guangzhou 510640, Guangdong, Peoples R China

期刊名称：HORTSCIENCE （ 影响因子：1.455； 五年影响因子：1.617 ）

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收录情况： SCI

摘要： MS is the most commonly used basal medium for the micropropagation of banana (Musa spp.), in which inorganic N source consists of NH_4~+-N and NO_3~--N, and NH_4~+:NO_3~- = 1:2. In the present study, basal medium for rooting culture was modified by supplying NO_3~--N as the sole N source at the concentration of 17.80 to 19.78 mmol centre dot L~(-1). Not only was the percentage of qualified plantlets higher than that of MS or 1/2 MS, but the cost for medium preparation (per liter) was about $0.6 lowerthan that of MS based on local retail price, for the concentration of N decreased from about 60 mmol centre dot L~(-1)to nearly 20 mmoMA The effects of four factors: sucrose (25 to 35 g centre dot L~(-1)), NAA (0.2 to 1.0 mg centre dot L~(-1)), IBA (0.2to 1.0 mg centre dot L~(-1)), and basal medium with modified N source on the percentage of qualified plantlets were studied by using orthogonal design. The variance analysis of data showed that all the four factors significantly affected the process ofrooting culture. Among them, sucrose was the most important factor, followed by revised basal medium, IBA, and NAA. The optimal medium for rooting culture was BM II supplemented with 30 g centre dot L~(-1) sucrose, 0.5 mg centre dot L~(-1) NAA, and 0.5 mg centre dot L~(-1) IBA. During the following acclimation phase, >90% of plantlets survived. Chemical name used: 6-benzylaminopurine (BA); indole-3-buryric acid (IBA); alpha-naphthaleneacetic acid (NAA).