文献类型： 外文期刊

作者： Pang, Y ¹ ; Shen, G ² ; Qi, H ² ; Tan, E ² ; Sun, X ² ; Tang, K ² ;

作者机构： 1.Fudan Univ, Sch Life Sci, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Morgan Tan Int Ctr Life Sci,State Key Lab Genet E, Shanghai 200433, Peoples R China

2.Fudan Univ, Sch Life Sci, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Morgan Tan Int Ctr Life Sci,State Key Lab Genet E, Shanghai 200433, Peoples R China; Hubei Acad Agr Sci, Hybrid Rice Res Ctr, Wuhan 430064, Peoples R China; SW China Normal Univ, Fac Life Sci, Chongqing 400715, Peoples R China

期刊名称：ENGINEERING IN LIFE SCIENCES （ 影响因子：2.678； 五年影响因子：2.704 ）

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收录情况： SCI

摘要： Aphid is one of the most serious, sap-sucking insect pests which cause significant losses of crop yields. The aim of this study is to investigate whether transgenic plants expressing Zephyranthes Candida agglutinin (ZCA) could confer enhanced resistance to aphids. Tobacco was transformed with a plasmid, pCAMBIAZCA, containing the marker genes nptll and gusA and the Zephyranthes Candida agglutinin gene (zca) via Agrobacterium tumefaciens-mediated transformation. Twenty-six independent transgenic plants were regenerated. Western blot analysis revealed ZCA expression at various levels in transgenic plants. Insect bioassay tests showed that transgenic plants expressing a high level of ZCA significantly inhibited the growth of the population of peach potato aphids (Myzus persicae Sulzer). This is the first report in which transgenic plants expressing ZCA conferred enhanced resistance to aphids. Our study suggests the zca gene could be a useful candidate for genetic engineering strategies in plants to provide aphid resistance.